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Study on a Luminol-based Electrochemiluminescent Sensor for Label-Free DNA Sensing
Institute of Analytical Chemistry, Department of Chemistry, Soochow University, Dushu Lake Higher Education Town, China-Singapore Suzhou Industrial Park, Jiangsu, 215123, China
* Author to whom correspondence should be addressed.
Received: 18 August 2010; in revised form: 25 September 2010 / Accepted: 8 October 2010 / Published: 21 October 2010
Abstract: Automatic, inexpensive, simple and sensitive methods for DNA sensing and quantification are highly desirable for biomedical research. The rapid development of both the fundamentals and applications of electrochemiluminescence (ECL) over the past years has demonstrated its potential for analytical and bio-analytical chemistry. This paper reports the quenching effect of DNA on the ECL of luminol and the further development of a DNA sensing device. With the pre-functionalization by a composite of carbon nano-tubes (CNTs) and Au nanoparticles (AuNPs), the sensor provides a novel and valuable label-free approach for DNA sensing. Here the ECL intensity was remarkably decreased when more than 1.0 × 10−12 molar of DNA were adsorbed on the sensor. Linearity of the DNA amount with the reciprocal of ECL intensity was observed. A saturated sensor caused a 92.8% quenching effect. The research also proposes the mechanism for the quenching effect which could be attributed to the interaction between luminol and DNA and the elimination of reactive oxygen species (ROSs) by DNA.
Keywords: DNA; label-free sensing; electrochemiluminescence; nano-prefunctional sensor; quenching effect
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Chu, H.-H.; Yan, J.-L.; Tu, Y.-F. Study on a Luminol-based Electrochemiluminescent Sensor for Label-Free DNA Sensing. Sensors 2010, 10, 9481-9492.
Chu H-H, Yan J-L, Tu Y-F. Study on a Luminol-based Electrochemiluminescent Sensor for Label-Free DNA Sensing. Sensors. 2010; 10(10):9481-9492.
Chu, Hai-Hong; Yan, Ji-Lin; Tu, Yi-Feng. 2010. "Study on a Luminol-based Electrochemiluminescent Sensor for Label-Free DNA Sensing." Sensors 10, no. 10: 9481-9492.