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Genetic Diversity of Black Salamanders (Aneides flavipunctatus) across Watersheds in the Klamath Mountains
Museum of Vertebrate Zoology and Department of Integrative Biology, University of California, 3101 Valley Life Sciences Building, Berkeley, CA 94720-3160, USA
Department of Ecology and Evolutionary Biology, University of California, 1156 High Street, Santa Cruz, CA 95064, USA
Bureau of Land Management, Medford Interagency Office, Medford, OR 97504, USA
Museu Nacional, Departamento de Vertebrados, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, RJ 20940-040, Brazil
* Author to whom correspondence should be addressed.
Received: 31 May 2013; in revised form: 2 August 2013 / Accepted: 8 August 2013 / Published: 29 August 2013
Abstract: Here we characterize the genetic structure of Black Salamanders (Aneides flavipunctatus) in the Klamath Mountains of northwestern California and southwestern Oregon using mitochondrial and nuclear DNA sequences. We hypothesized that the Sacramento, Smith, Klamath, and Rogue River watersheds would represent distinct genetic populations based on prior ecological results, which suggest that Black Salamanders avoid high elevations such as the ridges that separate watersheds. Our mitochondrial results revealed two major lineages, one in the Sacramento River watershed, and another containing the Klamath, Smith, and Rogue River watersheds. Clustering analyses of our thirteen nuclear loci show the Sacramento watershed population to be genetically distinctive. Populations in the Klamath, Smith, and Rogue watersheds are also distinctive but not as differentiated and their boundaries do not correspond to watersheds. Our historical demographic analyses suggest that the Sacramento population has been isolated from the Klamath populations since the mid-Pleistocene, with negligible subsequent gene flow (2 Nm ≤ 0.1). The Smith and Rogue River watershed populations show genetic signals of recent population expansion. These results suggest that the Sacramento River and Klamath River watersheds served as Pleistocene refugia, and that the Rogue and Smith River watersheds were colonized more recently by northward range expansion from the Klamath.
Keywords: amphibians; historical demography; mitochondrial DNA; nuclear DNA; phylogeography; population genetics
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Reilly, S.B.; Mulks, M.F.; Reilly, J.M.; Jennings, W.B.; Wake, D.B. Genetic Diversity of Black Salamanders (Aneides flavipunctatus) across Watersheds in the Klamath Mountains. Diversity 2013, 5, 657-679.
Reilly SB, Mulks MF, Reilly JM, Jennings WB, Wake DB. Genetic Diversity of Black Salamanders (Aneides flavipunctatus) across Watersheds in the Klamath Mountains. Diversity. 2013; 5(3):657-679.
Reilly, Sean B.; Mulks, Mitchell F.; Reilly, Jason M.; Jennings, W. B.; Wake, David B. 2013. "Genetic Diversity of Black Salamanders (Aneides flavipunctatus) across Watersheds in the Klamath Mountains." Diversity 5, no. 3: 657-679.