- freely available
Diversity 2013, 5(2), 374-392; doi:10.3390/d5020374
Published: 21 May 2013
Abstract: Although constituting more than 100,000 described species, protists are virtually ignored within the arena of biodiversity conservation. One reason is the widespread belief that the majority of protists have cosmopolitan distributions, in contrast to the highly hetereogenous biogeography of the “mega-Metazoa”. However, modern research reveals that about one third of the known protists have restricted distributions, which endorses their conservation, at least in special cases. Here, we report what probably ranks as the first successful conservation intervention focused directly on known protist diversity. It is justified by unique species, type localities, and landscape maintenance as evidence for legislation. The protected habitat comprises an ephemeral pond, which is now a “Natural Monument” for ciliated protozoa. This wetland occupies a natural depression on the Krauthügel (“cabbage hill”) south of the fortress of Salzburg City. When filled, the claviform pond has a size of ~30 × 15 m and a depth rarely surpassing 30 cm. Water is present only for some days or weeks, depending on heavy and/or prolonged rain. The pond occupied an agricultural field where root and leafy vegetables were cultivated for possibly more than 200 years. In the 1960s, this area became a grassland utilized as an autumn pasture, but was abandoned in the 1990s. Repeated sampling between 1982 and 2012 recovered a total of at least 150 ciliate taxa, of which 121 were identified to species level. Eight species were new to science, and an additional 10 poorly known species were reinvestigated and neotypified with populations from the Krauthügel pond. Both endemism and type localities justify the argument that the “integrative approach” in biodiversity and conservation issues should include protists and micro-metazoans. We argue that Krauthügel holds a unique reference node for biodiversity inventories to obtain the baseline knowledge—which is the prerequisite to monitor ecosystem integrity—and detect and evaluate impacts of natural and anthropogenic disturbances.
Rarely, if ever, do we consider microscopic organisms, such as protists and small pluricellular eukaryotes (e.g., rotifers, mosses), in conservation issues. Microscopic inconspicuousness is one obvious reason for their neglect. An academic argument justifies least concern for these “micro-biota”. This assumes the known species have cosmopolitan distributions [1,2,3]. In contrast, biogeogeographical knowledge of plants, vertebrates and the better known Insecta (e.g., Lepidoptera, Odonata) is not only more comprehensive and precise; but a high degree of endemism is well documented in such “Mega-Metazoa”, and it is not surprising that protected area design has been informed primarily by these surrogate taxa [4,5]. However, two recent books [6,7] on the distribution and biogeography of microscopic organisms demonstrate convincingly that their biogeographical patterns approximate those of Metazoa. About one third of mapped species exhibit endemism, confirming the moderate endemicity model . Accordingly, biodiversity conservation should include these minute but genetically highly diverse organisms, especially in the framework of “integrative approaches” (for reviews, see [3,5]).
The major issues in protist conservation have been discussed previously [4,9]. At least one protist species becomes extinct with each metazoan lost to extinction; change or loss of habitats; loss of type localities; obviously, extinction of a species, and/or clade, equates to the irreversible loss of unique genetic resources. Extreme losses of this evolutionary potential can be gauged against the ancient history of protists, given that the start of the Eukaryotic radiation is estimated at ~1,400 Ma, in the mid-Proterozoic, in response to unique biogeochemical opportunities inaugurated on Earth’s first supercontinent .
Ultimately, in conservation biology, practical success subsumes theory. Here we report on a successful case of conservation of the ciliates of an ephemeral pond in the town of Salzburg. This pond is type locality for 18 ciliate species, but was suddenly filled with earth in the course of an outdoor art project. When we recognized this crisis in mid-2010, we applied for conservation, and succeeded in convincing the landowner to remove the earth. We hope that our effort will stimulate colleagues to try the same for other small habitats with great diversity of certain groups of protists and/or microscopic pluricellular organisms.
2. Study Area and Methods
2.1. The Krauthügel Pond
The Krauthügel pond is at the southern foot of the fortress of Hohensalzburg, i.e., near the center of the town of Salzburg at the northeast margin of estate 2324 KG, E13°02'40.3'' N47°47'30.7'', 429.7 m above the Adriatic Sea. Here is a small agricultural area on a hill called “Krauthügel” (cabbage hill). The temporary pond occupies a natural depression overgrown by herbaceous vegetation, except in the barren center (Figure 1, Figure 2). Water is present only for some days or weeks, depending on short, but heavy, rains (mainly thunderstorms) or long periods of rain. When filled, the pond is clavate and has a size of about 30 × 15 m and a depth rarely surpassing 30 cm (Figure 1).
The Krauthügel has experienced a shifting history of landuse. It occupied an agricultural field where root and leafy vegetables (e.g., cabbage) were cultivated between 1789 and 1960. Then, it became a pasture for cows, whose excrements strongly eutrophied the pond water. This caused population blooms of a diversity of autotrophic and heterotrophic protists. Grazing of the pasture ceased after 1990. In medieval times, the pond was possibly influenced by an open canal (Alm canal) that supplied the town with drinking water. Most recently in the 20th century, expansion of the road network and urbanization culminated in landscape fragmentation to isolate the Krauthügel from the local drainage network. Taken together, the historical evidence indicates that the natural landscape depression produced an ephemeral pond of variable size and structure over several centuries. Although the geomorphology of this portion of the landscape has not been studied directly, the Krauthügel appears to be an abandoned stream infilled by surface runoff, and anthropogenic activities appear to have contributed to its formation. The original drainage channel and depression was likely modified in the Middle Ages with expansion of agriculture in the environs of the medieval town.
We investigated the ciliates of the Krauthügel pond sporadically between the years 1982 and 2012, using orthodox collection methods. The most intense investigation periods were the years 1982–1983; 2005–2007 when Meseres corlissi, one of the new species discovered in the pond, was subject of a focal research program of the Austrian Science Fund ; and 2010–2012 when the conservation procedure started.
Limnetic habitats: Plankton (mesh size 12 µm) and bottom mud; mud and water from cow steps and the agricultural machinery; infusions set up with the material collected and often enriched by some squashed wheat grains.
Soil-water habitats: During the Science Fund project, about 70 soil samples (0–10 cm) from the pond margin were investigated for ciliates, using the non-flooded Petri dish method described by Foissner . Briefly, this simple method involves placing 20–500 g of air-dried soil in a Petri dish (13–18 cm wide, 2–3 cm high) and saturating, but not flooding it, with distilled water. These cultures were analyzed for ciliates by inspecting about 2 ml of the run-off on days 2, 7, 14, 21, and 28.
The ciliates of all samples were first inspected in vivo. When there was indication that an unknown or poorly known species was present with sufficient abundance, it was investigated using the methods described by Foissner , i.e., detailed live observation, various silver impregnation techniques, and scanning electron microscopy. Preparations of these species were deposited in a museum (for details, see Chapter 3.1.).
3. Faunistics of the Ciliate Community of the Krauthügel Pond
3.1. Remarkable Species
All holotypes, paratypes, neotypes and vouchers have been deposited in the Biologiezentrum of the Oberösterreichische Landesmuseum in Linz (Biology Centre of the Museum of Upper Austria, Linz (LI). Present curator: Dr. Erna Aescht; e-mail: email@example.com). The taxa are ordered alphabetically.
Acropisthium mutabile Perty, 1852 . A highly variable, possibly globally distributed haptorid ciliate whose identity is threatened. Thus, it is here neotypified with protargol-impregnated specimens deposited by Foissner : inv. nos. 1984/ 44, 45.
Actinorhabdos trichocystiferus Foissner, 1984  (Figure 3b). An inconspicuous haptorid ciliate with highly distinct, awl-shaped extrusomes first described from the Krauthügel pond: inv. nos. 1984/ 29, 30 (protargol impregnation). Recently re-discovered in a litter and soil sample from the restinga on the outskirt of the town of Rio de Janeiro, Brazil.
Apertospathula implicata (Kahl, 1930)  Foissner & Oertel 2009 . One of the “difficult” spathidiid haptorids redescribed and neotypified by Foissner & Oertel : inv. nos. 2013/ 28–32 (protargol impregnation). A very rare, microaerobic species as yet reported only from Germany (pond in the botanical garden of the town of Hamburg) and the Krauthügel pond. Thus, possibly restricted to the holarctic.
Apodileptus visscheri visscheri Dragesco, 1963 . A “difficult” dileptid haptorid redescribed and neotypified by Vďačný & Foissner , using a population from the Krauthügel pond: inv. nos. 2011/ 218–225 (protargol impregnation). A voucher population from Lower Austria has the inv. nos. 2011/ 210–217. This is the nominal subspecies which differs by the shape of the extrusomes from A. visscheri rhabdoplites Vďačný & Foissner, 2012.
Enchelyodon anulatus (Foissner, 1984)  (Figure 3d). A highly characteristic haptorid ciliate with several globular macronuclear nodules each having a ring-shaped nucleolus. First described from the Krauthügel pond: inv. nos: 1984/ 42, 1984/ 43 (protargol impregnation). No other record known.
Frontonia angusta angusta (Kahl, 1931) . The nominal subspecies has been redescribed and neotypified by Foissner et al. , using the population from the Krauthügel pond: inv. nos. 2002/ 628–630 (Chatton- Lwoff silver nitrate impregnation).
Furgasonia theresae (Fabre- Domergue, 1889)  Foissner et al., 2002 . This nassulid ciliate has been neotypified by Foissner et al. , based on the population from the Krauthügel pond described by Foissner : inv. nos. 1988/ 29, 1988/ 30 (Chatton- Lwoff silver nitrate impregnation).
Fuscheria nodosa salisburgensis Gabilondo & Foissner, 2009  (Figure 3g). A new haptorid subspecies first described by Gabilondo & Foissner  from the Krauthügel pond: inv. nos. 2010/52–61. No other record known.
Meseres corlissi Petz & Foissner, 1992  (Figure 3a). This oligotrich ciliate was discovered in the Krauthügel pond: inv. nos. 1993/ 52, 1993/ 53 (protargol impregnation). It served as an example for ciliate distribution in a research program of the Austrian Science Fund . Likely a cosmopolitan.
Perispira ovum Stein, 1859 . A rare haptorid ciliate that will be redescribed and neotypified with the population from the Krauthügel pond.
Protospathidium serpens (Kahl, 1930)  Foissner, 1981 . This haptorid ciliate was re- studied and neotypified by Xu & Foissner : inv. nos. 2007/ 133–135 (protargol impregnation). It is a common mud and soil ciliate composed of cryptic species differing in the morphology of the resting cyst.
Pseudouroleptus procerus Berger & Foissner, 1987  (Figure 3d). This hypotrich ciliate was discovered in a soil sample from the margin of the Krauthügel pond: inv. nos. 1986/ 61, 1986/ 62. Later recorded from the Palaeotropis .
Psilotricha succisa (O. F. Müller, 1786)  Foissner, 1983 . Redescription and ontogenesis by Foissner . Identity threatened by similar populations from Japan and Africa. Thus, here neotypified with the population from the Krauthügel pond: inv. nos. 2013/ 20–24 (protargol impregnation).
Tillina magna Gruber, 1880 . This species belongs to the class Colpodea. Recent morphological and molecular studies showed the existence of several similar species threaten the identity of the Gruber species. Thus, we neotypify the species with specimens from the Krauthügel pond (slide 1984/ 28 Chatton- Lwoff silver nitrate method and with Figures 148r–y in Foissner , based on material from the Krauthügel pond.
3.2. Species Numbers and Community Structure
Over 30 years, we recorded about 150 ciliate taxa, of which 121 were identified to species level (Table 1). Ten of the 121 species were originally undescribed but two of them have been later described from other sites (Bilamellophrya hawaiiensis, Coriplites grandis), leaving the seven species shown in Figure 3 and at least one further new species among the unidentified ones (Table 1). Thus, so far, 10 new ciliate taxa have been discovered in this small, ephemeral pond.
Five species have been previously neotypified from populations of the Krauthügel pond, and five further species are neotypified in the present study or will be neotypified in forthcoming studies (Acropisthium mutabile, Maryna umbrellata, Perispira ovum, Psilotricha succisa, Tillina magna). Thus, the Krauthügel pond is type locality for 18 ciliate species. Additionally, seven species were redescribed from the Krauthügel pond (Table 1).
The ciliate community of the Krauthügel pond is a mixture of species from stagnant and permanent running waters (e.g., Amphileptus pleurosigma, Cinetochilum margaritaceum, Euplotes affinis, Paramecium caudatum, Steinia sphagnicola, Uroleptus gallina), soil and moss (e.g., Colpoda maupasi, Drepanomonas pauciciliata, Euplotes muscicola, Grossglockneria spp., Mykophagophrys terricola, Spathidium claviforme), and ephemeral waters (Bresslaua spp., Bursaria truncatella, Furgasonia spp., Maryna spp., Opisthonecta spp., Tillina magna), the latter belonging to the classes Colpodea (Bresslaua, Bursaria, Maryna, Tillina), Nassophorea (Furgasonia) and Oligohymenophorea (Opisthonecta).
|Table 1. Ciliate species recorded from the Krauthügel-pond.|
|*||Acropisthium mutabile Perty, 1852|
|Actinorhabdos trichocystiferus Foissner, 1984|
|Amphileptus pleurosigma (Stokes, 1884) Foissner, 1984|
|**||Apertospathula implicata (Kahl, 1930) Foissner & Oertel, 2009|
|**||Apodileptus visscheri visscheri (Dragesco, 1963) Vďaĉný & Foissner, 2012|
|Arcuospathidium cooperi Foissner, 1996|
|Askenasia volvox (Eichwald, 1852) Kahl, 1930|
|Aspidisca cicada (Mueller, 1786) Claparède & Lachmann, 1858|
|Balantidion pellucidum Eberhard, 1862|
|Bilamellophrya hawaiensis Foissner, Agatha & Berger, 2002|
|Blepharisma steini Kahl, 1932|
|Blepharisma undulans Stein, 1867|
|Bresslaua insidiatrix Claff, Dewey & Kidder, 1941|
|Bryometopus atypicus Foissner, 1980|
|Bryometopus pseudochilodon Kahl, 1932|
|Bursaria truncatella Mueller, 1773|
|Bursellopsis truncata (Kahl, 1927) Corliss, 1960|
|Chilodonella uncinata (Ehrenberg, 1838) Strand, 1928|
|Chilodontopsis depressa (Perty, 1852) Blochmann, 1895|
|Cinetochilum margaritaceum (Ehrenberg, 1831) Perty, 1849|
|Colpoda aspera Kahl, 1926|
|Colpoda cucullus (Mueller, 1773) Gmelin, 1790|
|Colpoda inflata (Stokes, 1884) Kahl, 1931|
|Colpoda lucida Greeff, 1888|
|Colpoda maupasi Enriques, 1908|
|Colpodidium bradburyarum Foissner, Agatha & Berger, 2002|
|Colpodidium caudatum Wilbert, 1982|
|Colpodidium microstoma Foissner, Agatha & Berger, 2002|
|Coriplites grandis Oertel et al., 2008|
|Cyclidium glaucoma Mueller, 1773|
|Cyrtolophosis minor Vuxanovici, 1963|
|***||Cyrtolophosis mucicola Stokes, 1885|
|Deviata bacilliformis (Gelei, 1954) Eigner, 1995|
|***||Didinium nasutum (Mueller, 1773) Stein, 1859|
|***||Dileptus anatinus Golińska, 1971|
|Dileptus margaritifer (Ehrenberg, 1833) Dujardin, 1841|
|Drepanomonas pauciciliata Foissner, 1987|
|***||Enchelydium piliforme (Kahl, 1930) Foissner, 1984|
|Enchelyodon anulatus Foissner, 1984|
|Engelmanniella mobilis (Engelmann, 1862) Foissner, 1982|
|Epispathidium ascendens (Wenzel, 1955) Foissner, 1987|
|Epistylis alpestris Foissner, 1978|
|Euplotes affinis (Dujardin, 1841) Kahl, 1932|
|Euplotes muscicola Kahl, 1932|
|**||Frontonia angusta angusta Kahl, 1931|
|Furgasonia rubens (Perty, 1852) Jankowski, 1964|
|**||Furgasonia theresae (Fabre-Domergue, 1889) Foissner, Agatha & Berger, 2002|
|Fuscheria nodosa salisburgensis Gabilondo & Foissner, 2009|
|Gastrostyla mystacea mystacea (Stein, 1859) Sterki, 1878|
|Grossglockneria acuta Foissner, 1980|
|Grossglockneria hyalina Foissner, 1985|
|***||Halteria grandinella (Mueller, 1773) Dujardin, 1841|
|Holophrya teres (Ehrenberg, 1833) Foissner, Berger & Kohmann, 1994|
|Holosticha stueberi Foissner, 1987|
|Homalogastra setosa Kahl, 1926|
|Kahliella simplex (Horváth, 1934) Corliss, 1960|
|Kahlilembus attenuatus (Smith, 1897) Foissner, Berger & Kohmann, 1994|
|Leptopharynx costatus Mermod, 1914|
|Maryna lichenicola (Gelei, 1950) Foissner, 1993|
|***||Maryna ovata (Gelei, 1950) Foissner, 1993|
|*||Maryna umbrellata (Gelei, 1950) Foissner, 1993|
|Meseres corlissi Petz & Foissner, 1992|
|Metopus hasei Sondheim, 1929|
|Metopus inversus (Jankowski, 1964) Foissner & Agatha, 1999|
|Metopus minor Kahl, 1927|
|Metopus palaeformis Kahl, 1927|
|Monodinium balbiani Fabre-Domergue, 1888|
|Monomacrocaryon terrenum (Foissner, 1981) Vďaĉný et al., 2012|
|Mykophagophrys terricola (Foissner, 1985) Foissner, 1995|
|Odontochlamys alpestris Foissner, 1981|
|Opisthonecta minima Foissner, 1975|
|Opisthonecta bivacuolata Foissner, 1978|
|Oxytricha opisthomuscorum Foissner et al., 1991|
|Papillorhabdos multinucleatus Foissner, 1984|
|Paracolpoda steinii (Maupas, 1883) Lynn, 1978|
|Paramecium caudatum Ehrenberg, 1833|
|Paramphisiella caudata (Hemberger, 1985) Foissner, 1988|
|*||Perispira ovum Stein, 1859|
|Plagiocampa difficilis Foissner, 1981|
|Platyophrya vorax Kahl, 1926|
|Podophrya fixa (Mueller, 1786) Ehrenberg, 1833|
|Protocyclidium terrenum Alekperov, 1993|
|**||Protospathidium serpens (Kahl, 1930) Foissner, 1981|
|**||Pseudochilodonopsis algivora (Kahl, 1931) Foissner, 1979|
|Pseudochilodonopsis piscatoris (Blochmann, 1895) Foissner, 1979|
|Pseudomicrothorax dubius (Maupas, 1883) Penard, 1922|
|Pseudoplatyophrya nana (Kahl, 1926) Foissner, 1980|
|Pseudouroleptus procerus Berger & Foissner, 1987|
|Pseudourostyla franzi Foissner, 1987|
|***||Psilotricha succisa (Mueller, 1786) Foissner, 1983|
|Rostrophryides africana Foissner, 1987|
|Sagittaria hyalina Foissner, Czapik & Wiakowski. 1981|
|Sathrophilus muscorum (Kahl, 1931) Corliss, 1960|
|Semispathidium pulchrum Foissner, Hess & Al-Rasheid, 2010|
|Spathidium claviforme Kahl, 1930|
|Spathidium procerum Kahl, 1930|
|Spathidium puteolagri Baumeister in Kahl (1930)|
|Spathidium rusticanum Foissner, 1981|
|Spathidium spathula (Mueller, 1773) Moody, 1912|
|Steinia platystoma (Ehrenberg, 1831) Diesing, 1866|
|Steinia sphagnicola Foissner, 1989|
|Sterkiella histriomuscorum (Foissner, Blatterer, Berger &|
|Kohmann, 1991) Foissner, Blatterer, Berger & Kohmann, 1991|
|Strobilidium caudatum (Fromentel, 1876) Foissner, 1987|
|Stylonychia notophora Stokes, 1885|
|Tetrahymena rostrata (Kahl, 1926) Corliss, 1952|
|*||Tillina magna Gruber, 1880|
|Tokophrya infusionum (Stein, 1859) Bütschli, 1889|
|Uroleptus gallina (Mueller, 1786) Foissner, Blatterer, Berger & Kohmann, 1991|
|Urosoma acuminata (Stokes, 1887) Kahl, 1932|
|Urosoma emarginata (Stokes, 1885) Berger, 1999|
|Urostyla grandis Ehrenberg, 1830|
|Urotricha farcta Claparède & Lachmann, 1859|
|Urotricha globosa Schewiakoff, 1892|
|Vorticella (Echinovorticella) echini (King, 1931) Foissner, Agatha & Berger, 2002|
|Vasicola ciliata Tatem, 1869|
|Woodruffides metabolicus (Johnson & Larson, 1938) Foissner, 1987|
|Epistylis sp. n.?|
|Notohymena sp. n.?|
|Odontochlamys n. sp.?|
|Spathidium -like gen. n., sp. n. (description in preparation)|
|Urotricha sp. n.?|
|Epispathidium cf. amphoriforme|
Bold = new species, * species neotypified in this paper, ** species neotypified previously, *** redescribed species. References to the authors and combining authors of the species are provided in the literature section but only when it is a new, a neotypified, or a redescribed species. For most others, this information can be found in the reviews of Foissner  and Foissner et al. [27,46,47].
Four of the ten new ciliate taxa from the Krauthügel pond have also been recorded from other biogeographic regions: Actinorhabdos trichocystiferus was re-discovered in a soil sample on the outskirts of Rio de Janeiro (Foissner, unpubl.); Bilamellophrya hawaiiensis has been discovered in arable soil from Hawaii ; Coriplites grandis was discovered in soil south of the town of Cairns, Australia ; and Meseres corlissi has been recorded from China, Australia, Africa, and South America .
3.4. Official Recognition
After two positive reviews, our application was accepted by the mayor of the town of Salzburg. On 19.01.2012 and 15.02.2012, he declared the “Ephemeral pond on the Krauthügel” as a “Natural Monument for Single-celled Organisms”, protecting the pond and a buffer zone (Figure 4), altogether an area of 5,290 m2 on plot 2324 KG .
4.1. Number of Ciliate Species in Other Well-Investigated Ephemeral Waters
Studies of the kind presented are very rare because ephemeral waters are not very attractive for limnologists and well-trained ciliate taxonomists were always an exception. Further, the data are hardly comparable because of great differences in the number of waters investigated, the number of samples taken, the time range samples were studied, and the methods used for ciliate identification.
Nonetheless, a considerable amount of data has been accumulated (for a review, see ), and thus we put together the most similar investigations in Table 2. This shows that the total number of species in the Krauthügel pond is in the upper range, possible because of the high age of the pond and the detailed investigations, while the percentage of new species is in the middle range, possibly because Bresslau , Gelei [33,52], Gruber , Dingfelder , and Foissner  have already described many species characteristic for ephemeral waters in central and eastern Europe. Our unpublished data from ephemeral waters of Venezuela and Australia indicate that many species wait to be discovered [30,62].
|Table 2. Number of ciliate species in well-investigated ephemeral waters.|
|Brief site description||Numberof species||% newspecies||Authors|
|1.||Many ephemeral waters sampled across Central Europe a||69||10|||
|2.||Five ephemeral, eutrophic pasture ponds, each investigated several times during two years, on a mountain meadow in Hungary||75||45||[52,53,54]|
|3.||785 ephemeral waters, mainly road and meadow puddles in Bavaria, Germany||132||12|||
|4.||An arctic tundra pond sampled several times b||~30||0?|||
|5.||23 alpine, eutrophic pasture ponds in Austria, most investigated 1 to 2 times (but see next entry)||107||33|||
|6.||Many samples from pasture pond 1 in the series mentioned above||54||17|||
|7.||Four soil samples from a seasonally inundated grassland in New Zealand||57||0?|||
|8.||Many samples from an experimental ricefield in Italy studied for four years||60||0?|||
|9.||One sample from a few road puddles within the Bambatsi guest house area (Namibia, Southwest Africa)||130||14|||
|10.||Two meltwater ponds in southern Ontario (Canada) investigated weekly for 98 and 34 days, respectively||145||0?|||
|11.||Nine Lajas (granitic lithotelmes in Venezuela), each investigated one time (Foissner, unpubl.)||103||9|
4.2. Conservation Arguments
Usually, conservation appeals are based on the narrow distribution of one or several species and their habitat, or of species and habitats endangered by human activities . In the Krauthügel case, five of the 10 new species have not been reported from any other locality. Although we cannot exclude future discoveries in other biogeographic regions, presently they have to be considered as endemics. Accordingly, the likely endemism of some of these new species recorded only in the Krauthügel habitat was accepted by the reviewers of our application.
4.2.2. Type Locality and Landscape Diversity
The epistemological credentials of the Type Locality  underpins the second main argument in our application to conserve the Krauthügel. Small, ephemeral waterlands are especially prone to being “flattened” by anthropogenic agencies, because they occupy landforms that decrease the economic value of farmland. In case of the Krauthügel, this would mean the loss of the type localities of eight new species and 10 neotype localities.
The type locality is the site where a previously unknown organism was first discovered and redescribed for the first time ; it is critical to taxonomy as the geographical place location of capture or collection of the name-bearing type of a nominal species or subspecies. For protists and several small pluricellular eukaryotes, the type locality is much more important than in larger organisms because their presence and distribution cannot be seen with the naked eye but needs complex instruments and methods. Unfortunately, habitat destruction, which is responsible for one third of biodiversity loss globally , has erased many protist type localities. In ciliates, the biggest loss occurred by draining of the wetland on the outskirts of the town of Hamburg (Germany), where Alfred Kahl discovered more than hundred new species . In their epistemological impacts on scientific knowledge, loss of type localities and their populations characterize immediate depredations of the Second Alexandrian Tragedy—the extirpation of biological knowledge [66,67]. Anthropogenic threats to biotic habitats that qualify as type localities constitute poorly appreciated conservation targets. Obviously, the future integrity of the natural science collections will vouch for the biotic uniqueness of the Krauthügel.
When the type specimen has been lost or not deposited, as for most protists, the identity of the species may be threatened forever. Therefore, a neotype should be established . The site of the neotype becomes the new type locality . Thus, the Krauthügel pond qualifies as the type locality for eight new species and for 10 neotypified species. This criteria and value of the type locality extends to future research, when we acknowledge that phylotyping  of these known ciliates will provide an essential benchmark for future explorations of cryptic and ‘unculturable’ biodiversity in the Krauthügel.
We note this landform occupies a very restricted natural setting, but argue that the merits of preserving it outweigh impacts of human interference on the natural environment. The position and context of the Krauthügel does raise questions as to what extent humans should interfere technologically, or otherwise, with natural environmental processes to ensure persistence of a natural ecosystem that otherwise would likely be altered or obliterated by not only anthropogenic but equally natural processes. Here, a buffer zone has been established, largely to prevent incursions by human activity, but it has nonetheless altered natural geomorphological processes that can be expected to have altered the site. There was a heavy loss of small permanent and ephemeral waters during the past fifty years when agriculture became industrialized. This had many negative consequences, of which the loss of local biodiversity and monotonization of landscapes were the most serious ones . Without the pond, the Krauthügel, which is heavily used by walkers, would be exposed to both of these undesirable impacts.
Conservation is frequently associated with limited agriculture and thus financial loss for the owner. In the Krauthügel case, this played no role because the protected area and the buffer zone should be farmed as usual. Accordingly, the problems discussed by Hansen and De Fries  do not apply.
4.3. A Benchmark for Future Biodiversity Characterization
Legislated preservation of the Krauthügel prioritizes the landform’s microbiota as a keystone target for metagenomic surveys [71,72]. Phylotyping  will characterize the known species of protozoa in the context of the sympatric cryptic and ‘unculturable’ biodiversity. Such an exercise will constitute an inaugural landmark in a research strategy that integrates new discoveries of microbial diversity with published knowledge. We argue for an expansion of this exercise beyond its inaugural application to the Krauthügel.
Focal metagenomic characterizations of the type localities of protists (Table 2) across landscapes will open the way for a scientifically robust strategy to quantify the known versus unknown microbial diversity. This approach can profitably integrate rarefaction methods  to quantify new discoveries of cryptic taxa. Metagenomic characterization of formally described microbial taxa (pertinently ciliates) will provide the benchmark to evaluate regional, continental and global patterns of microbial biodiversity. Moreover, repeated metagenomic surveys of these focal and associated habitats will provide the foundation of data detailing the intricacies of ecosystem dynamics, which are barely explored, let alone, understood. Such baseline knowledge of biodiversity is the prerequisite for future monitoring, to detect and evaluate impacts of natural and anthropogenic disturbances within and across the landscape mosaics in which keystone habitats are embedded. As argued by Cotterill et al. , the importance of this strategy is underscored by the key roles of ciliates (and protists) in nearly all ecosystems, notably as predators in food webs, and especially in agroecolandscapes and hydrological systems.
4.4. Taxonomy Informs Conservation
There is an increasing number of studies that argue for preservation of microbial diversity [4,74,75]. Further, there are many studies that find a “high molecular diversity” in a variety of habitats. Unfortunately, most of these studies did not include morphological investigations leaving the species unnamed. However, in practice, conservation decisions need named species because these reflect refined knowledge of biodiversity (indeed, the species list was important for the Krauthügel project, and its knowledge continues to improve). Thus, we fully support the appeal of Mace  that solving conservation issues need a more intimate collaboration among conservation biologists, taxonomists and legislators, but, above all, increased investment in taxonomists with relevant and high-quality skills. High quality scientific taxonomy should inform conservation decisions and not the other way around .
We have invoked evolutionary novelty (local endemism), preservation of taxonomic knowledge (type locality), and landscape maintenance as arguments for the conservation of a small, ephemeral pond. Although its spatial extent is relatively miniscule when considered against established protected area networks, this does not undermine its relevance to ecosystem management and biodiversity research. Considerable ciliate endemism and a focal type locality are the criteria of biotic and epistemic uniqueness, respectively, which underpins the conversation arguments. Legislation that conserves the Krauthügel serves to maintain the ecological integrity of a benchmark habitat. The case of the Krauthügel exemplifies a previously unappreciated role of such benchmark landforms as repositories of focal data. They hold a key role in integrated research to advance the frontiers of knowledge of microbial biodiversity. Arguments for monitoring programmes that seek to quantify ecosystem integrity further underscore the values of maintaining high fidelity knowledge of microbial habitats.
Financial support was given to WF by the Austrian Science Fund (Projects P20360–B17 and P22846–B17). The technical assistance of Mag. Barbara Harl and Johannes Rattey MSc is greatly acknowledged. We also greatly acknowledge the owner of the Krauthügel, i.e., the convent of St. Peter, and several politicians, especially Johann Padutsch, a councilor of the town of Salzburg. We thank the referees for constructive feedback.
- Finlay, B.J.; Corliss, J.O.; Esteban, G.; Fenchel, T. Biodiversity at the microbial level: the number of free-living ciliates in the biosphere. Q. Rev. Biol. 1996, 71, 221–237. [Google Scholar]
- Finlay, B.J.; Esteban, G.F.; Fenchel, T. Protist diversity is different? Protist 2004, 155, 15–22. [Google Scholar] [CrossRef]
- Markert, B.A.; Breure, A.M.; Zechmeister, H.G. Bioindicators & Biomonitors Principles, Concepts and Applications; Elsevier: Amsterdam, the Netherlands, 2003; p. 997. [Google Scholar]
- Cotterill, F.P.D.; Al-Rasheid, K.; Foissner, W. Conservation of protists: Is it needed at all? Biodivers. Conserv. 2008, 17, 427–443. [Google Scholar] [CrossRef]
- Likens, G.E.; Lindenmayer, D.B. Integrating approaches leads to more effective conservation of biodiversity. Biodivers. Conserv. 2012, 21, 3323–3341. [Google Scholar] [CrossRef]
- Foissner, W.P. Diversity and Geographical Distribution. Biodivers. Conserv. 2008, 17, 235–343. [Google Scholar] [CrossRef]
- Fontaneto, D. Biogeography of Microscopic Organisms. Is Everything Small Everywhere?; Cambridge University Press: Cambridge, UK, 2011; p. 365. [Google Scholar]
- Foissner, W. Protist diversity and distribution: Some basic considerations. Biodivers. Conserv. 2008, 17, 235–242. [Google Scholar] [CrossRef]
- Cotterill, F.P.D.; Foissner, W. A pervasive denigration of natural history misconstrues how biodiversity inventories and taxonomy underpin scientific knowledge. Biodivers. Conserv. 2010, 19, 291–303. [Google Scholar] [CrossRef]
- Parnell, J.; Hole, M.; Boyce, A.J.; Spinks, S.; Bowden, S. Heavy metal, sex and granites: Crustal differentiation and bioavailability in the mid-Proterozoic. Geology 2012, 40, 751–754. [Google Scholar] [CrossRef]
- Weisse, T.; Strüder-Kypke, C.; Berger, H.; Foissner, W. Genetic, morphological, and ecological diversity of spatially separated clones of Meseres corlissi Petz & Foissner, 1992 (Ciliophora, Spirotrichea). J. Eukaryot. Microbiol. 2008, 55, 257–270. [Google Scholar] [CrossRef]
- Foissner, W. Soil protozoa: fundamental problems, ecological significance, adaptations in ciliates and testaceans, bioindicators, and guide to the literature. Progr. Protistol. 1987, 2, 69–212. [Google Scholar]
- Foissner, W. Basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa. Europ. J. Protistol. 1991, 27, 313–330. [Google Scholar] [CrossRef]
- Perty, M. Zur Kenntniss kleinster Lebensformen nach Bau, Funktionen, Systematik, mit Specialverzeichnis der in der Schweiz beobachteten. Jent & Reinert: Bern, Switzerland, 1852; p. 228. [Google Scholar]
- Foissner, W. Infraciliatur, Silberliniensystem und Biometrie einiger neuer und wenig bekannter terrestrischer, limnischer und mariner Ciliaten (Protozoa: Ciliophora) aus den Klassen Kinetofragminophora, Colpodea und Polyhymenophora. Stapfia 1984, 12, 1–165. [Google Scholar]
- Kahl, A. Neue und ergänzende Beobachtungen holotricher Infusorien. II. Arch. Protistenk. 1930, 70, 313–416. [Google Scholar]
- Foissner, W.; Oertel, A. Morphology and ciliary pattern of some rare haptorid ciliates, with a description of the new family Kamburophryidae (Protists, Haptoria). Europ. J. Protistol. 2009, 45, 205–218. [Google Scholar] [CrossRef]
- Dragesco, J. Revision du genre Dileptus., Dujardin 1871 (Ciliata Holotricha) (systématique, cytologie, biologie). Bull. Biol. Fr. Belg. 1963, 97, 103–145. [Google Scholar]
- Vd’ačný, P.; Foissner, W. Monograph of the dileptids (Protista, Ciliophora, Rhynchostomatia). Denisia 2012, 31, 1–529. [Google Scholar]
- Stokes, A.C. Some new infusoria. Am. Nat. 1885, 19, 433–443. [Google Scholar]
- Foissner, W. Neue und wenig bekannte hypotriche und colpodide Ciliaten (Protozoa: Ciliophora) aus Böden und Moosen. Zool. Beitr. N. F. 1987, 31, 187–282. [Google Scholar]
- Müller, O.F. Vermium Terrestrium Et Fluviatilium, Seu Animalium Infusorium, Helminthicorum Et Testaceorum, Non Marinorum, Succincta Historia. Heineck & Faber: Havniae, Denmark & Lipsiae, Germany, 1773; p. 135. [Google Scholar]
- Stein, F. Charakteristik neuer Infusorien-Gattungen. Lotos 1859, 9, 2–5. [Google Scholar]
- Golińska, K. Comparative studies on the morphology of Dileptus anatinus sp. n. (Holotricha, Gymnostomata). Acta. Protozool. 1971, 8, 367–378. [Google Scholar]
- Kahl, A. Urtiere oder Protozoa I: Wimpertiere oder Ciliata (Infusoria) 1. Allgemeiner Teil und Prostomata. Tierwelt. Dtl. 1930, 18, 1–180. [Google Scholar]
- Kahl, A. Urtiere oder Protozoa I: Wimpertiere oder Ciliata (Infusoria) 2. Holotricha außer den im 1. Teil behandelten Prostomata. Tierwelt. Dtl. 1931, 21, 181–398. [Google Scholar]
- Foissner, W.; Agatha, S.; Berger, H. Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa), with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Denisia 2002, 5, 1–1459. [Google Scholar]
- Fabre-Domergue, P.L. Sur une nouvelle forme de colpode (Colpoda. henneguyi) et sur un flagellé pélagique. Annls. Microgr. 1889, 2, 351–357. [Google Scholar]
- Foissner, W. Morphologie und Infraciliatur einiger neuer und wenig bekannter terrestrischer und limnischer Ciliaten (Protozoa, Ciliophora). Sber. Akad. Wiss. Wien. 1989, 196, 173–247. [Google Scholar]
- Gabilondo, R.; Foissner, W. Four new fuscheriid soil ciliates (Ciliophora: Haptorida) from four biogeographic regions. Acta. Protozool. 2009, 48, 1–24. [Google Scholar]
- Dujardin, F. Histoire Naturelle Des Zoophytes Infusoire; Suites à Buffon: Paris, France, 1841; p. 684. [Google Scholar]
- Petz, W.; Foissner, W. Morphology and morphogenesis of Strobilidium. caudatum (Fromentel), Meseres corlissi n. sp., Halteria grandinella (Müller), and Strombidium rehwaldi n. sp., and a proposed phylogenetic system for oligotrichid ciliates (Protozoa, Ciliophora). J. Protozool. 1992, 39, 159–176. [Google Scholar]
- Gelei, J. Die Marynidae der Sodagewässer in der Nähe von Szeged XIV Beitrag zur Ziliatenfauna Ungarns. Hidrol. Közl. 1950, 30, 107–119, 157–158. [Google Scholar]
- Foissner, W. Protozoenfauna. Volume 4/1: Colpodea (Ciliophora.); Fischer: Stuttgart, Germany, 1993; pp. I–X, 798. [Google Scholar]
- Stein, F. Charakteristik neuer Infusorien-Gattungen. Lotos 1859, 9, 57–60. [Google Scholar]
- Foissner, W. Morphologie und Taxonomie einiger neuer und wenig bekannter kinetofragminophorer Ciliaten (Protozoa: Ciliophora) aus alpinen Böden. Zool. Jb. Syst. 1981, 108, 264–297. [Google Scholar]
- Xu, K.; Foissner, W. Descriptions of Protospathidium serpens (Kahl, 1930) and P. fraterculum n. sp. (Ciliophora, Haptoria), two species based on different resting cyst morphology. J. Eukaryot. Microbiol. 2005, 52, 298–309. [Google Scholar] [CrossRef]
- Foissner, W. Ökologische und systematische Studien über das Neuston alpiner Kleingewässer, mit besonderer Berücksichtigung der Ciliaten. Int. Revue ges. Hydrobiol. 1979, 64, 99–140. [Google Scholar] [CrossRef]
- Foissner, W. Taxonomie und Ökologie einiger Ciliaten (Protozoa, Ciliophora) des Saprobiensystems. II. Familie Chilodonellidae. Hydrobiologia 1988, 162, 21–45. [Google Scholar] [CrossRef]
- Berger, H.; Foissner, W. Morphology and biometry of some soil hypotrichs (Protozoa: Ciliophora). Zool. Jb. Syst. 1987, 114, 193–239. [Google Scholar]
- Foissner, W. An updated compilation of world soil ciliates (Protozoa, Ciliophora), with ecological notes, new records, and descriptions of new species. Europ. J. Protistol. 1998, 34, 195–235. [Google Scholar] [CrossRef]
- Müller, O.F. Animalcula Infusoria Fluviatilia Et Marina, Quae Detexit, Systematice Descripsit Et Ad Vivum Delineari Curavit; N. Mölleri: Hauniae, Denmark, 1786; p. 367. [Google Scholar]
- Foissner, W. Morphologie und Morphogenese von Psilotricha succisa (O.F. Müller, 1786) nov. comb. (Ciliophora, Hypotrichida). Protistologica 1983, 19, 479–493. [Google Scholar]
- Foissner, W.; Hess, S.; Al-Rasheid, K.A.S. Two vicariant Semispathidium species from tropical Africa and central Europe: S. fraterculum nov. spec. and S. pulchrum nov. spec. (Ciliophora, Haptorida). Europ. J. Protistol. 2010, 46, 61–73. [Google Scholar] [CrossRef]
- Gruber, A. Neue Infusorien. Z. Wiss. Zool. 1880, 33, 439–466. [Google Scholar]
- Foissner, W.; Berger, H.; Blatterer, H.; Kohmann, F. Taxonomische und Ökologische Revision der Ciliaten des Saprobiensystems Band I–IV; Bayerisches Landesamtes für Wasserwirtschaft: Deggendorf, Germany, 1995; pp. 1–540. [Google Scholar]
- Foissner, W.; Berger, H.; Schaumburg, J. Identification and Ecology of Limnetic Plankton Ciliates; Bayerisches Landesamtes für Wasserwirtschaft: Deggendorf, Germany, 1999; p. 793. [Google Scholar]
- Oertel, A.; Wolf, K.; Al-Rasheid, K.; Foissner, W. Revision of the genus Coriplites Foissner, 1988 (Ciliophora: Haptorida), with description of Apocoriplites nov. gen. and three new species. Acta. Protozool. 2008, 47, 231–246. [Google Scholar]
- Stadt Salzburg. Amtsblatt 2012, 4, 1–7.
- Williams, D.D. The Biology of Temporary Waters; Oxford University Press: New York, NY, USA, 2006; p. 337. [Google Scholar]
- Spandl, H. Die Tierwelt vorübergehender Gewässer Mitteleuropas. Arch. Hydrobiol. 1926, 16, 74–132. [Google Scholar]
- Gelei, J. Über die Lebensgemeinschaft einiger temporärer Tümpel auf einer Bergwiese im Börzsönygebirge (Oberungarn) III Ciliaten. Acta. biol. hung. 1954, 5, 259–343. [Google Scholar]
- Gelei, J. Über die Lebensgemeinschaft einiger temporärer Tümpel auf einer Bergwiese im Börzsönygebirge (Oberungarn) I Die Tümpel. Acta. biol. hung. 1954, 5, 227–233. [Google Scholar]
- Gelei, J.; Megyeri, J.; Szabados, M.; Varga, L. Über die Lebensgemeinschaft einiger temporärer Tümpel auf einer Bergwiese im Börzsönygebirge (Oberungarn) VIII Allgemeine Betrachtungen. Acta. biol. hung. 1954, 5, 363–382. [Google Scholar]
- Dingfelder, J.H. Die Ciliaten vorübergehender Gewässer. Arch. Protistenk 1962, 105, 509–658. [Google Scholar]
- Fenchel, T. The quantitative importance of the benthic microfauna of an arctic tundra pond. Hydrobiologia. 1975, 46, 445–464. [Google Scholar] [CrossRef]
- Foissner, W. Artenbestand und Struktur der Ciliatenzönose in alpinen Kleingewässern (Hohe Tauern, Österreich). Arch. Protistenk. 1980, 123, 99–126. [Google Scholar] [CrossRef]
- Stout, J.D. The protozoan fauna of a seasonally inundated soil under grassland. Soil Biol. Biochem. 1984, 16, 121–125. [Google Scholar] [CrossRef]
- Madoni, P. The contribution of ciliated protozoa to plankton and benthos biomass in a European ricefield. J. Eukaryot. Microbiol. 1996, 43, 193–198. [Google Scholar] [CrossRef]
- Andrushchyshyn, O.; Magnusson, A.K.; Williams, D.D. Ciliate populations in temporary freshwater ponds: seasonal dynamics and influential factors. Freshwater Biol. 2003, 48, 548–564. [Google Scholar] [CrossRef]
- Bresslau, E. Über Protozoen aus Rasenaufgüssen. Verh. dt. zool. Ges. 1922, 27, 88–90. [Google Scholar]
- Foissner, W.; Kreutz, M. Systematic position and phylogenetic relationships of the genera Bursaridium, Paracondylostoma, Thylakidium, Bryometopus., and Bursaria. (Ciliophora: Colpodea). Acta Protozool. 1998, 37, 227–240. [Google Scholar]
- Townsend, C.R.; Harper, J.L.; Begon, M.E. Ökologie; Springer: Berlin, Germany, 2003; p. 647. [Google Scholar]
- ICZN (The International Commission on Zoological Nomenclature). International Code of Zoological Nomenclature; International Trust for Zoological Nomenclature: London, UK, 1999; p. 306.
- Foissner, W.; Wenzel, F. Life and legacy of an outstanding ciliate taxonomist, Alfred Kahl (1877–1946), including a facsimile of his forgotten monograph from 1943. Acta. Protozool. 2004, 43 (Suppl.), 3–69. [Google Scholar]
- Cotterill, F.P.D. Systematics, biological knowledge and environmental conservation. Biodivers. Conserv. 1995, 4, 183–205. [Google Scholar] [CrossRef]
- Cotterill, F.P.D. The Second Alexandrian Tragedy, and the fundamental relationship between biological collections and scientific knowledge. In The Values and Valuation of Natural Science Collections, Proceedings of Conference at the Manchester Museum, Manchester, UK, 19–21 April 1995; Nudds, J.R., Pettitt, C.R., Eds.; Geological Society: London, UK; pp. 217–241.
- Foissner, W. Neotypification of protists, especially ciliates (Protozoa, Ciliophora). Bull. Zool. Nom. 2002, 59, 165–169. [Google Scholar]
- Wu, D.; Wu, M.; Halpern, A.; Rusch, D.B.; Yooseph, S.; Frazier, M.; Venter, J.C.; Eisen, J.A. Stalking the fourth domain in metagenomic data: searching for, discovering, and interpreting novel, deep branches in marker gene phylogenetic trees. PLoS One 2011, 6, e18011. [Google Scholar]
- Hansen, A.J.; DeFries, R. Ecological mechanisms linking protected areas to surrounding lands. Ecol. Appl. 2007, 17, 974–988. [Google Scholar] [CrossRef]
- Handelsman, J.; Rondon, M.R.; Brady, S.F.; Clardy, J.; Goodman, R.M. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem. Biol. 1998, 5, R245–R249. [Google Scholar] [CrossRef]
- Morgan, J.L.; Darling, A.E.; Eisen, J.A. Metagenomic sequencing of an in vitro-simulated microbial community. PLoS One 2010, 5, e10209. [Google Scholar] [CrossRef]
- Foissner, W.; Chao, A.; Katz, L.A. Diversity and geographic distribution of ciliates (Protista: Ciliophora). Biodivers. Conserv. 2008, 17, 345–363. [Google Scholar] [CrossRef]
- Barberán, A.; Casamayor, E.O. Euxinic freshwater hypolimnia promote bacterial endemicity in continental areas. Microb. Ecol. 2011, 61, 465–472. [Google Scholar] [CrossRef]
- Griffith, G.W. Do we need a global strategy for microbial conservation? Trends Ecol. Evol. 2012, 27, 1–2. [Google Scholar] [CrossRef]
- Mace, G.M. The role of taxonomy in species conservation. Phil. Trans. R. Soc. Lond. B. 2004, 359, 711–719. [Google Scholar] [CrossRef]
- Bernardo, J. A critical appraisal of the meaning and diagnosability of cryptic evolutionary diversity, and its implications for conservation in the face of climate change. In Climate Change, Ecology and Systematics; Hodkinson, T.R., Jones, M.B., Waldren, S., Parnell, J.A.N., Eds.; Cambridge University Press, The Systematics Association: Cambridge, UK, 2011; pp. 380–438. [Google Scholar]
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