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The Effect of Tillage System and Crop Rotation on Soil Microbial Diversity and Composition in a Subtropical Acrisol
Department of Soil Science, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA
* Author to whom correspondence should be addressed.
Received: 29 August 2012; in revised form: 11 October 2012 / Accepted: 19 October 2012 / Published: 31 October 2012
Abstract: Agricultural management alters physical and chemical soil properties, which directly affects microbial life strategies and community composition. The microbial community drives important nutrient cycling processes that can influence soil quality, cropping productivity and environmental sustainability. In this research, a long-term agricultural experiment in a subtropical Acrisol was studied in south Brazil. The plots at this site represent two tillage systems, two nitrogen fertilization regimes and three crop rotation systems. Using Illumina high-throughput sequencing of the 16S rRNA gene, the archaeal and bacterial composition was determined from phylum to species level in the different plot treatments. The relative abundance of these taxes was correlated with measured soil properties. The P, Mg, total organic carbon, total N and mineral N were significantly higher in the no-tillage system. The microbial diversity was higher in the no-tillage system at order, family, genus and species level. In addition, overall microbial composition changed significantly between conventional tillage and no-tillage systems. Anaerobic bacteria, such as clostridia, dominate in no-tilled soil as well as anaerobic methanogenic archaea, which were detected only in the no-tillage system. Microbial diversity was higher in plots in which only cereals (oat and maize) were grown. Soil management influenced soil biodiversity on Acrisol by change of composition and abundance of individual species.
Keywords: microbial community; microbial diversity; tillage system; high-throughput sequencing; 16S rRNA gene; soil
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Dorr de Quadros, P.; Zhalnina, K.; Davis-Richardson, A.; Fagen, J.R.; Drew, J.; Bayer, C.; Camargo, F.A.; Triplett, E.W. The Effect of Tillage System and Crop Rotation on Soil Microbial Diversity and Composition in a Subtropical Acrisol. Diversity 2012, 4, 375-395.
Dorr de Quadros P, Zhalnina K, Davis-Richardson A, Fagen JR, Drew J, Bayer C, Camargo FA, Triplett EW. The Effect of Tillage System and Crop Rotation on Soil Microbial Diversity and Composition in a Subtropical Acrisol. Diversity. 2012; 4(4):375-395.
Dorr de Quadros, Patricia; Zhalnina, Kateryna; Davis-Richardson, Austin; Fagen, Jennie R.; Drew, Jennifer; Bayer, Cimelio; Camargo, Flavio A.O.; Triplett, Eric W. 2012. "The Effect of Tillage System and Crop Rotation on Soil Microbial Diversity and Composition in a Subtropical Acrisol." Diversity 4, no. 4: 375-395.