Diversity 2010, 2(4), 572-585; doi:10.3390/d2040572
Article

cTBP: A Successful Intron Length Polymorphism (ILP)-Based Genotyping Method Targeted to Well Defined Experimental Needs

Luca Bragliaemail, Antonella Mancaemail, Francesco Mastromauroemail and Diego Breviario* email
Institute of Agricultural Biology and Biotechnology (IBBA) -C.N.R Via Bassini 15, 20133 Milan, Italy
* Author to whom correspondence should be addressed.
Received: 20 February 2010; in revised form: 2 April 2010 / Accepted: 8 April 2010 / Published: 15 April 2010
(This article belongs to the Special Issue Biological Diversity Assessed by Molecular Methods)
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Abstract: There seem to be a certain degree of reluctance in accepting ILP-based methods as part of the range of molecular markers that are classically used for plant genotyping. Indeed, since DNA polymorphism results from difference in length of fragments amplified from specific gene loci, not anonymous sequences, the number of markers that can be generated is sometime inadequate for classical phylogeny studies. Yet, ILP-based markers have many other useful advantages that should not go neglected. We support this statement by presenting a large variety of data we have been collecting for a long while regarding the use of cTBP, an ILP marker based on difference in length of the introns present within the members of the plant beta-tubulin gene family.
Keywords: plant genotyping; molecular markers; intron length polymorphism; plant β-tubulin

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MDPI and ACS Style

Braglia, L.; Manca, A.; Mastromauro, F.; Breviario, D. cTBP: A Successful Intron Length Polymorphism (ILP)-Based Genotyping Method Targeted to Well Defined Experimental Needs. Diversity 2010, 2, 572-585.

AMA Style

Braglia L, Manca A, Mastromauro F, Breviario D. cTBP: A Successful Intron Length Polymorphism (ILP)-Based Genotyping Method Targeted to Well Defined Experimental Needs. Diversity. 2010; 2(4):572-585.

Chicago/Turabian Style

Braglia, Luca; Manca, Antonella; Mastromauro, Francesco; Breviario, Diego. 2010. "cTBP: A Successful Intron Length Polymorphism (ILP)-Based Genotyping Method Targeted to Well Defined Experimental Needs." Diversity 2, no. 4: 572-585.

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