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Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints
U.R. Agro-biodiversity, Higher Agronomic Institute, 4042 Chott Mariem, Sousse, Tunisia
Laboratory of Molecular Genetics, Immunology & Biotechnology, Faculty of Sciences of Tunis, Campus University, 2092, El Manar, Tunis, Tunisia
Laboratory of Biochemistry & Molecular Biology, Faculty of Pharmacy, 5019 Monastir, Tunisia
* Author to whom correspondence should be addressed.
Received: 16 December 2009; Accepted: 12 January 2010 / Published: 18 January 2010
Abstract: The genetic diversity among Tunisian pomegranate cultivars has been investigated. Using universal primers, the random amplified polymorphic DNA (RAPD) method was used to generate banding profiles from a set of twelve cultivars. Data was then computed with appropriate programs to construct a dendrogram illustrating the relationships between the studied cultivars. Our data proved the efficiency of the designed method to examine the DNA polymorphism in this crop since the tested primers are characterized by a collective resolving power of 12.83. In addition, the cluster analysis has exhibited a parsimonious tree branching independent from the geographic origin of the cultivars. In spite of the relatively low number of primers and cultivars, RAPD constitutes an appropriate procedure to assess the genetic diversity and to survey the phylogenetic relationships in this crop.
Keywords: pomegranate; Punica granatum L.; diversity; RAPD
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Hasnaoui, N.; Mars, M.; Chibani, J.; Trifi, M. Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints. Diversity 2010, 2, 107-114.
Hasnaoui N, Mars M, Chibani J, Trifi M. Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints. Diversity. 2010; 2(1):107-114.
Hasnaoui, Néjib; Mars, Messaoud; Chibani, Jemni; Trifi, Mokhtar. 2010. "Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints." Diversity 2, no. 1: 107-114.