Int. J. Mol. Sci. 2008, 9(5), 906-913; doi:10.3390/ijms9050906
Article

Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum

Konstantinos M. Kasiotis1,* email, Helen Souki2 , Angelos N. Tsakirakis1 , Haris Carageorgiou2 email, Spiridon A. Theotokatos3 email, Serkos A. Haroutounian4 email and Kyriaki Machera1 email
1 Benaki Phytopathological Institute, Laboratory of Pesticides Toxicology, 8 St. Delta Street, Kifissia 14561, Athens, Greece 2 Pharmacology Department, Medical School, University of Athens, 115 27, Athens, Greece 3 Agroindustrial Robola Growers Cooperative of Kefalllinia, Omala, 28100 Kefallinia, Greece 4 Chemistry Laboratory, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece
* Author to whom correspondence should be addressed.
Received: 15 April 2008; in revised form: 22 May 2008 / Accepted: 23 May 2008 / Published: 23 May 2008
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Abstract: A simple and effective analytical procedure was developed for the determination of fenthion residues in human serum samples. The sample treatment was performed using the headspace solid-phase micro extraction with polyacrylate fiber, which has the advantage to require low amount of serum (1 mL) without tedious pre-treatment. The quantification of fenthion was carried out by gas chromatography-mass spectrometry and the recoveries ranged from 79 to 104% at two spiking levels for 6 replicates. Detection and quantification limits were calculated as 1.51 and 4.54 ng/mL of serum respectively. Two fenthion metabolites − fenoxon and fenthion–sulfoxide − were also identified.
Keywords: Insecticide residue; olive; fenthion; headspace; SPME; GC/MS; metabolites.

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MDPI and ACS Style

Kasiotis, K.M.; Souki, H.; Tsakirakis, A.N.; Carageorgiou, H.; Theotokatos, S.A.; Haroutounian, S.A.; Machera, K. Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum. Int. J. Mol. Sci. 2008, 9, 906-913.

AMA Style

Kasiotis K.M., Souki H., Tsakirakis A.N., Carageorgiou H., Theotokatos S.A., Haroutounian S.A., Machera K. Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum. International Journal of Molecular Sciences. 2008; 9(5):906-913.

Chicago/Turabian Style

Kasiotis, Konstantinos M.; Souki, Helen; Tsakirakis, Angelos N.; Carageorgiou, Haris; Theotokatos, Spiridon A.; Haroutounian, Serkos A.; Machera, Kyriaki. 2008. "Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum." Int. J. Mol. Sci. 9, no. 5: 906-913.

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