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Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum
Benaki Phytopathological Institute, Laboratory of Pesticides Toxicology, 8 St. Delta Street, Kifissia 14561, Athens, Greece
Pharmacology Department, Medical School, University of Athens, 115 27, Athens, Greece
Agroindustrial Robola Growers Cooperative of Kefalllinia, Omala, 28100 Kefallinia, Greece
Chemistry Laboratory, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece
* Author to whom correspondence should be addressed.
Received: 15 April 2008; in revised form: 22 May 2008 / Accepted: 23 May 2008 / Published: 23 May 2008
Abstract: A simple and effective analytical procedure was developed for the determination of fenthion residues in human serum samples. The sample treatment was performed using the headspace solid-phase micro extraction with polyacrylate fiber, which has the advantage to require low amount of serum (1 mL) without tedious pre-treatment. The quantification of fenthion was carried out by gas chromatography-mass spectrometry and the recoveries ranged from 79 to 104% at two spiking levels for 6 replicates. Detection and quantification limits were calculated as 1.51 and 4.54 ng/mL of serum respectively. Two fenthion metabolites − fenoxon and fenthion–sulfoxide − were also identified.
Keywords: Insecticide residue; olive; fenthion; headspace; SPME; GC/MS; metabolites.
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Kasiotis, K.M.; Souki, H.; Tsakirakis, A.N.; Carageorgiou, H.; Theotokatos, S.A.; Haroutounian, S.A.; Machera, K. Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum. Int. J. Mol. Sci. 2008, 9, 906-913.
Kasiotis KM, Souki H, Tsakirakis AN, Carageorgiou H, Theotokatos SA, Haroutounian SA, Machera K. Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum. International Journal of Molecular Sciences. 2008; 9(5):906-913.
Kasiotis, Konstantinos M.; Souki, Helen; Tsakirakis, Angelos N.; Carageorgiou, Haris; Theotokatos, Spiridon A.; Haroutounian, Serkos A.; Machera, Kyriaki. 2008. "Headspace Solid Phase Micro Extraction Gas Chromatographic Determination of Fenthion in Human Serum." Int. J. Mol. Sci. 9, no. 5: 906-913.