Int. J. Mol. Sci. 2008, 9(4), 662-667; doi:10.3390/ijms9040662
A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA
1
Laboratory of Single-Molecule Biophysics and Polymer Physics, Clemson University, Clemson, SC 29634-0978, USA
2
Carl Zeiss MicroImaging, Inc. One Zeiss Drive, Thornwood, NY 10594, USA
*
Author to whom correspondence should be addressed.
Received: 21 December 2007 / Revised: 22 April 2008 / Accepted: 23 April 2008 / Published: 24 April 2008
(This article belongs to the Special Issue Nucleic Acid Derivatives in Emerging Technologies)
Abstract
The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possibly due to strand crosslinking. The structural damage of DNA was further examined using transmission electron microscopy which revealed kinks and sharp bends along the DNA backbone.Keywords:
DNA damage; UV radiation; flexibility; diffusion.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Schallhorn, K.; Kim, M.; Ke, P.C. A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA. Int. J. Mol. Sci. 2008, 9, 662-667.
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