Next Article in Journal / Special Issue
CELL-SELEX: Novel Perspectives of Aptamer-Based Therapeutics
Previous Article in Journal
Specific siRNA Targeting the Receptor for Advanced Glycation End Products Inhibits Experimental Hepatic Fibrosis in Rats
Previous Article in Special Issue
Effect of Locked-Nucleic Acid on a Biologically Active G-Quadruplex. A Structure-Activity Relationship of the Thrombin Aptamer
Article Versions
Related Info
More by Authors
Share This Article
  •  Twitter
  •  Facebook
  •  Mendeley
  •  CiteULike
  • More services
Int. J. Mol. Sci. 2008, 9(4), 662-667; doi:10.3390/ijms9040662
Communication

A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA

1,2, 1 and 1,*
Received: 21 December 2007 / Revised: 22 April 2008 / Accepted: 23 April 2008 / Published: 24 April 2008
(This article belongs to the Special Issue Nucleic Acid Derivatives in Emerging Technologies)
View Full-Text   |   Download PDF [577 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possibly due to strand crosslinking. The structural damage of DNA was further examined using transmission electron microscopy which revealed kinks and sharp bends along the DNA backbone.
Keywords: DNA damage; UV radiation; flexibility; diffusion. DNA damage; UV radiation; flexibility; diffusion.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Schallhorn, K.; Kim, M.; Ke, P.C. A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA. Int. J. Mol. Sci. 2008, 9, 662-667.

AMA Style

Schallhorn K, Kim M, Ke PC. A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA. International Journal of Molecular Sciences. 2008; 9(4):662-667.

Chicago/Turabian Style

Schallhorn, Kathryn; Kim, Mickey; Ke, Pu C. 2008. "A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA." Int. J. Mol. Sci. 9, no. 4: 662-667.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert