Next Article in Journal / Special Issue
CELL-SELEX: Novel Perspectives of Aptamer-Based Therapeutics
Previous Article in Journal
Specific siRNA Targeting the Receptor for Advanced Glycation End Products Inhibits Experimental Hepatic Fibrosis in Rats
Previous Article in Special Issue
Effect of Locked-Nucleic Acid on a Biologically Active G-Quadruplex. A Structure-Activity Relationship of the Thrombin Aptamer
Int. J. Mol. Sci. 2008, 9(4), 662-667; doi:10.3390/ijms9040662

A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA

1,2, 1 and 1,*
1 Laboratory of Single-Molecule Biophysics and Polymer Physics, Clemson University, Clemson, SC 29634-0978, USA 2 Carl Zeiss MicroImaging, Inc. One Zeiss Drive, Thornwood, NY 10594, USA
* Author to whom correspondence should be addressed.
Received: 21 December 2007 / Revised: 22 April 2008 / Accepted: 23 April 2008 / Published: 24 April 2008
(This article belongs to the Special Issue Nucleic Acid Derivatives in Emerging Technologies)
View Full-Text   |   Download PDF [577 KB, uploaded 19 June 2014]   |   Browse Figures


The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possibly due to strand crosslinking. The structural damage of DNA was further examined using transmission electron microscopy which revealed kinks and sharp bends along the DNA backbone.
Keywords: DNA damage; UV radiation; flexibility; diffusion. DNA damage; UV radiation; flexibility; diffusion.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Schallhorn, K.; Kim, M.; Ke, P.C. A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA. Int. J. Mol. Sci. 2008, 9, 662-667.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert