This article is
- freely available
Vasorelaxant and Antioxidant Activities of Spilanthes acmella Murr.
Department of Pharmacology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand
Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand
Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand
Laboratory of Pharmacology, Chulabhorn Research Institute, Bangkok 10210, Thailand
Laboratory of Medicinal Chemistry, Chulabhorn Research Institute , Bangkok 10210, Thailand
* Authors to whom correspondence should be addressed.
Received: 12 June 2008; in revised form: 14 December 2008 / Accepted: 14 December 2008 / Published: 18 December 2008
Abstract: This study reports the effect of Spilanthes acmella Murr. extracts on phenylephrine-induced contraction of rat thoracic aorta as well as their antioxidant activity. Results show that the extracts exert maximal vasorelaxations in a dose-dependent manner, but their effects are less than acetylcholine-induced nitric oxide (NO) vasorelaxation. Significant reduction of vasorelaxations is observed in both NG-nitro-L-arginine methyl ester (L-NAME) and indomethacin (INDO). In the presence of L-NAME plus INDO, synergistic effects are observed, leading to loss of vasorelaxation of both acetylcholine and the extracts. Similarly, the vasorelaxations of the extracts are completely abolished upon the removal of endothelial cells. This demonstrates that the extracts exhibit vasorelaxation via partially endothelium-induced NO and prostacyclin in a dose-dependent manner. Significantly, the ethyl acetate extract exerts immediate vasorelaxation (ED50 76.1 ng/mL) and is the most potent antioxidant (DPPH assay). The chloroform extract shows the highest vasorelaxation and antioxidation (SOD assay). These reveal a potential source of vasodilators and antioxidants.
Keywords: Spilanthes acmella Murr.; vasorelaxant; antioxidant activities; nitric oxide; prostacyclin; thoracic aorta
Article StatisticsClick here to load and display the download statistics.
Notes: Multiple requests from the same IP address are counted as one view.
Cite This Article
MDPI and ACS Style
Wongsawatkul, O.; Prachayasittikul, S.; Isarankura-Na-Ayudhya, C.; Satayavivad, J.; Ruchirawat, S.; Prachayasittikul, V. Vasorelaxant and Antioxidant Activities of Spilanthes acmella Murr.. Int. J. Mol. Sci. 2008, 9, 2724-2744.
Wongsawatkul O, Prachayasittikul S, Isarankura-Na-Ayudhya C, Satayavivad J, Ruchirawat S, Prachayasittikul V. Vasorelaxant and Antioxidant Activities of Spilanthes acmella Murr.. International Journal of Molecular Sciences. 2008; 9(12):2724-2744.
Wongsawatkul, Orapin; Prachayasittikul, Supaluk; Isarankura-Na-Ayudhya, Chartchalerm; Satayavivad, Jutamaad; Ruchirawat, Somsak; Prachayasittikul, Virapong. 2008. "Vasorelaxant and Antioxidant Activities of Spilanthes acmella Murr.." Int. J. Mol. Sci. 9, no. 12: 2724-2744.