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Tobacco OPBP1 Enhances Salt Tolerance and Disease Resistance of Transgenic Rice
Key Laboratory of Plant Pathology, Ministry of Agriculture; Department of Plant Pathology, China Agricultural University, Beijing 100193, China
* Author to whom correspondence should be addressed.
Received: 19 November 2008; in revised form: 4 December 2008 / Accepted: 9 December 2008 / Published: 11 December 2008
Abstract: Osmotin promoter binding protein 1 (OPBP1), an AP2/ERF transcription factor of tobacco, has been demonstrated to function in disease resistance and salt tolerance in tobacco. To increase stress tolerant capability of rice, we generated rice plants with an OPBP1 overexpressing construct. Salinity shock treatment with 250 mM NaCl indicated that most of the OPBP1 transgenic plants can survive, whereas the control seedlings cannot. Similar recovery was found by using the seedlings grown in 200 mM NaCl for two weeks. The OPBP1 transgenic and control plants were also studied for oxidative stress tolerance by treatment with paraquat, showing the transgenic lines were damaged less in comparison with the control plants. Further, the OPBP1 overexpression lines exhibited enhanced resistance to infections of Magnaporthe oryzae and Rhizoctonia solani pathogens. Gene expressing analysis showed increase in mRNA accumulation of several stress related genes. These results suggest that expression of OPBP1 gene increase the detoxification capability of rice.
Keywords: AP2/EREBP; salt tolerance; disease resistance; transcription factor; transgenic rice
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Cite This Article
MDPI and ACS Style
Chen, X.; Guo, Z. Tobacco OPBP1 Enhances Salt Tolerance and Disease Resistance of Transgenic Rice. Int. J. Mol. Sci. 2008, 9, 2601-2613.
Chen X, Guo Z. Tobacco OPBP1 Enhances Salt Tolerance and Disease Resistance of Transgenic Rice. International Journal of Molecular Sciences. 2008; 9(12):2601-2613.
Chen, Xujun; Guo, Zejian. 2008. "Tobacco OPBP1 Enhances Salt Tolerance and Disease Resistance of Transgenic Rice." Int. J. Mol. Sci. 9, no. 12: 2601-2613.