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TFIP11 Interacts with mDEAH9, an RNA Helicase Involved in Spliceosome Disassembly
University of Southern California School of Dentistry, Center for Craniofacial Molecular Biology, 2250 Alcazar Street, CSA room 103, Los Angeles, California 90033-1004, USA
* Author to whom correspondence should be addressed.
Received: 18 August 2008; in revised form: 31 October 2008 / Accepted: 3 November 2008 / Published: 4 November 2008
Abstract: Yeast proteins Ntr1, Ntr2 and Prp43 function in spliceosome disassembly. An Ntr1-Ntr2 protein complex recruits Prp43 to allow the removal of the lariat-intron in latestage RNA splicing activity. Based on amino-acid sequence similarities across species, TFIP11 and mDEAH9/Dhx15 have been identified as homologues of yeast Ntr1 and Prp43, respectively. The N-terminal region of TFIP11 contains a G-patch, which is a highly conserved domain of many RNA-processing proteins. TFIP11 displays a unique and characteristic subnuclear localization pattern, in close proximity to SC35 nuclear speckles. Transfected GFP-tagged mDEAH9 displays an evenly distributed nuclear localization and is excluded from the nucleoli; however when TFIP11 and mDEAH9 are co-transfected, both proteins colocalize to distinct nuclear speckles. These data show that TFIP11 recruits mDEAH9 suggesting that these two proteins have similar biological activities to their yeast counterparts.
Keywords: mDEAH9; Dhx15; G-patch; Ntr1; pre-mRNA splicing; Prp43; spliceosome disassembly; Spp382 and TFIP11
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Wen, X.; Tannukit, S.; Paine, M.L. TFIP11 Interacts with mDEAH9, an RNA Helicase Involved in Spliceosome Disassembly. Int. J. Mol. Sci. 2008, 9, 2105-2113.
Wen X, Tannukit S, Paine ML. TFIP11 Interacts with mDEAH9, an RNA Helicase Involved in Spliceosome Disassembly. International Journal of Molecular Sciences. 2008; 9(11):2105-2113.
Wen, Xin; Tannukit, Sissada; Paine, Michael L. 2008. "TFIP11 Interacts with mDEAH9, an RNA Helicase Involved in Spliceosome Disassembly." Int. J. Mol. Sci. 9, no. 11: 2105-2113.