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Elevation of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Expression in the Mouse Brain after Chronic Nonylphenol Exposure
School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221008, Jiangsu Province, P.R. China
Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Xuzhou Normal University, Xuzhou 221116, Jiangsu Province, P.R. China
* Author to whom correspondence should be addressed.
Received: 21 March 2008; in revised form: 9 October 2008 / Accepted: 13 October 2008 / Published: 24 October 2008
Abstract: The present study was performed to investigate the effects of chronic administration of nonylphenol (NP) on the expression of inflammation-related genes in the brains of mice. NP was given orally by gavages at 0, 50, 100, and 200 mg/kg/d. The expression of inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), was evaluated by immunohistochemistry and immunoblotting assays. The nitric oxide (NO) level and nitric oxide synthase (NOS) activity were also measured by biochemical analyses. The results showed that NP at a high dose (200 mg/kg/d) significantly increased the expression of iNOS and COX-2 in both the hippocampus and cortex. In parallel with the increase in iNOS expression, the NO level was significantly greater at the dose of 200 mg/kg/d, compared to the control. The activity of NOS was also increased in the brain of mice at the dose of 100 and 200 mg/kg/d. These findings demonstrate that NP may have the potential to induce the chronic inflammation or cause neurotoxicity in the mouse brain.
Keywords: Nonylphenol; iNOS; COX-2; NO; brain
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MDPI and ACS Style
Zhang, Y.-Q.; Mao, Z.; Zheng, Y.-L.; Han, B.-P.; Chen, L.-T.; Li, J.; Li, F. Elevation of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Expression in the Mouse Brain after Chronic Nonylphenol Exposure. Int. J. Mol. Sci. 2008, 9, 1977-1988.
Zhang Y-Q, Mao Z, Zheng Y-L, Han B-P, Chen L-T, Li J, Li F. Elevation of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Expression in the Mouse Brain after Chronic Nonylphenol Exposure. International Journal of Molecular Sciences. 2008; 9(10):1977-1988.
Zhang, Yan-Qiu; Mao, Zhen; Zheng, Yuan-Lin; Han, Bao-Ping; Chen, Ling-Tong; Li, Jing; Li, Fei. 2008. "Elevation of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Expression in the Mouse Brain after Chronic Nonylphenol Exposure." Int. J. Mol. Sci. 9, no. 10: 1977-1988.