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Int. J. Mol. Sci. 2008, 9(10), 1977-1988; doi:10.3390/ijms9101977

Elevation of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Expression in the Mouse Brain after Chronic Nonylphenol Exposure

1, 1,2, 2,* , 1,2, 2, 2 and 2
1 School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221008, Jiangsu Province, P.R. China 2 Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Xuzhou Normal University, Xuzhou 221116, Jiangsu Province, P.R. China
* Author to whom correspondence should be addressed.
Received: 21 March 2008 / Revised: 9 October 2008 / Accepted: 13 October 2008 / Published: 24 October 2008
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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The present study was performed to investigate the effects of chronic administration of nonylphenol (NP) on the expression of inflammation-related genes in the brains of mice. NP was given orally by gavages at 0, 50, 100, and 200 mg/kg/d. The expression of inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), was evaluated by immunohistochemistry and immunoblotting assays. The nitric oxide (NO) level and nitric oxide synthase (NOS) activity were also measured by biochemical analyses. The results showed that NP at a high dose (200 mg/kg/d) significantly increased the expression of iNOS and COX-2 in both the hippocampus and cortex. In parallel with the increase in iNOS expression, the NO level was significantly greater at the dose of 200 mg/kg/d, compared to the control. The activity of NOS was also increased in the brain of mice at the dose of 100 and 200 mg/kg/d. These findings demonstrate that NP may have the potential to induce the chronic inflammation or cause neurotoxicity in the mouse brain.
Keywords: Nonylphenol; iNOS; COX-2; NO; brain Nonylphenol; iNOS; COX-2; NO; brain
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zhang, Y.-Q.; Mao, Z.; Zheng, Y.-L.; Han, B.-P.; Chen, L.-T.; Li, J.; Li, F. Elevation of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Expression in the Mouse Brain after Chronic Nonylphenol Exposure. Int. J. Mol. Sci. 2008, 9, 1977-1988.

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