Next Article in Journal
Salting-out in Aqueous Solutions of Ionic Liquids and K3PO4: Aqueous Biphasic Systems and Salt Precipitation
Previous Article in Journal
3D-QSAR Study of Potent Inhibitors of Phosphodiesterase-4 Using a CoMFA Approach
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2007, 8(7), 723-735; doi:10.3390/i8070723

Electrochemical Recognition of Metalloproteins by Bromide-modified Silver Electrode - A New Method

1
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
2
Department of Chemistry, Faculty of Science, University of Tehran, Tehran, Iran
3
Department of Chemistry, University of Agriculture, Faisalabad, Pakistan
4
Department of Chemistry, Faculty of Science, University of Mazandaran, Babolsar, Iran
*
Author to whom correspondence should be addressed.
Received: 8 May 2007 / Revised: 9 July 2007 / Accepted: 19 July 2007 / Published: 25 July 2007
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [244 KB, uploaded 19 June 2014]   |  

Abstract

A bromide–modified silver electrode is reported, in the present study, to catalyzethe redox reactions of metalloproteins. This study describes that the bromide ions showvery good redox behavior with silver electrode. The cathodic and anodic peak potentialswere related to the concentration of bromide ions involved in making bromide-modifiedsilver electrode. The electrode reaction in the bromine solution was a diffusion-controlledprocess. Positive potential shift of the bromide ions was seen when different proteins wereadded to the solution using a silver electrode. New cathodic and anodic peaks wereobserved at different potential ranges for myoglobin, cytochrome c and catalase. A linearlyincreasing cathodic peak current of bromide ions was seen when the concentration ofsuperoxide dismutase was increased in the test solution. However, no change for albuminwas observed when its concentration was increased in the test solution. Present data provesour methodology as an easy-to-use analysis for comparing the redox potentials of differentmetalloproteins and differentiating the metallo- from non-metalloproteins. In this study, weintroduced an interesting method for bio-electrochemical analyses.
Keywords: Bromide ions; Mediator; Silver electrode; metalloprotein; Bio-electrochemistry Bromide ions; Mediator; Silver electrode; metalloprotein; Bio-electrochemistry
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rezaei-Zarchi, S.; Saboury, A.A.; Norouzi, P.; Hong, J.; Barzegar, A.; Ganjali, M.R.; Ghourchian, H.; Moosavi-Movahedi, A.A.; Javed, A.; Rostami, A.A. Electrochemical Recognition of Metalloproteins by Bromide-modified Silver Electrode - A New Method. Int. J. Mol. Sci. 2007, 8, 723-735.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top