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Electrochemical Recognition of Metalloproteins by Bromide-modified Silver Electrode - A New Method
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Department of Chemistry, Faculty of Science, University of Tehran, Tehran, Iran
Department of Chemistry, University of Agriculture, Faisalabad, Pakistan
Department of Chemistry, Faculty of Science, University of Mazandaran, Babolsar, Iran
* Author to whom correspondence should be addressed.
Received: 8 May 2007; in revised form: 9 July 2007 / Accepted: 19 July 2007 / Published: 25 July 2007
Abstract: A bromide–modified silver electrode is reported, in the present study, to catalyzethe redox reactions of metalloproteins. This study describes that the bromide ions showvery good redox behavior with silver electrode. The cathodic and anodic peak potentialswere related to the concentration of bromide ions involved in making bromide-modifiedsilver electrode. The electrode reaction in the bromine solution was a diffusion-controlledprocess. Positive potential shift of the bromide ions was seen when different proteins wereadded to the solution using a silver electrode. New cathodic and anodic peaks wereobserved at different potential ranges for myoglobin, cytochrome c and catalase. A linearlyincreasing cathodic peak current of bromide ions was seen when the concentration ofsuperoxide dismutase was increased in the test solution. However, no change for albuminwas observed when its concentration was increased in the test solution. Present data provesour methodology as an easy-to-use analysis for comparing the redox potentials of differentmetalloproteins and differentiating the metallo- from non-metalloproteins. In this study, weintroduced an interesting method for bio-electrochemical analyses.
Keywords: Bromide ions; Mediator; Silver electrode; metalloprotein; Bio-electrochemistry
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Rezaei-Zarchi, S.; Saboury, A.A.; Norouzi, P.; Hong, J.; Barzegar, A.; Ganjali, M.R.; Ghourchian, H.; Moosavi-Movahedi, A.A.; Javed, A.; Rostami, A.A. Electrochemical Recognition of Metalloproteins by Bromide-modified Silver Electrode - A New Method. Int. J. Mol. Sci. 2007, 8, 723-735.
Rezaei-Zarchi S, Saboury AA, Norouzi P, Hong J, Barzegar A, Ganjali MR, Ghourchian H, Moosavi-Movahedi AA, Javed A, Rostami AA. Electrochemical Recognition of Metalloproteins by Bromide-modified Silver Electrode - A New Method. International Journal of Molecular Sciences. 2007; 8(7):723-735.
Rezaei-Zarchi, Saeed; Saboury, Ali A.; Norouzi, Parviz; Hong, Jun; Barzegar, Abolfazl; Ganjali, Mohammad R.; Ghourchian, Hedayatollah; Moosavi-Movahedi, Ali A.; Javed, Aisha; Rostami, Abbas A. 2007. "Electrochemical Recognition of Metalloproteins by Bromide-modified Silver Electrode - A New Method." Int. J. Mol. Sci. 8, no. 7: 723-735.