Next Article in Journal
Irradiation Effect on Oxidative Condition and Tocopherol Content of Vegetable Oils
Next Article in Special Issue
Dinuclear Zinc (II) Complexes of Macrocyclic Polyamine Ligands Containing an Imidazolium Bridge: Synthesis, Characterization, and Their Interaction with Plasmid DNA
Previous Article in Journal
Branched-chain Amino Acid Biosensing Using Fluorescent Modified Engineered Leucine/Isoleucine/Valine Binding Protein
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2007, 8(6), 526-532; doi:10.3390/i8060526

A Centrifugation-based Method for Preparation of Gold Nanoparticles and its Application in Biodetection

Department of Biochemistry, Nanjing University, Jiangsu 210093, China
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
Author to whom correspondence should be addressed.
Received: 18 April 2007 / Revised: 11 May 2007 / Accepted: 15 May 2007 / Published: 13 June 2007
(This article belongs to the Special Issue Nucleic Acid Derivatives in Emerging Technologies)
View Full-Text   |   Download PDF [664 KB, uploaded 19 June 2014]   |  


Gold nanoparticles (AuNPs) have found widespread applications in lifesciences. While synthesis of monodispersed AuNPs has been fairly convenient by usingchemical reduction of chloroauric acid by sodium citrate, we found that AuNPs of highquality and high concentrations were not readily obtained via this method. As an example,we showed that monodispersed 13-nm AuNPs were readily synthesized at relatively lowconcentrations (e.g. 3.5 nM); in contrast, 13-nm AuNPs of 17 nM obtained by the directreduction method were irregularly shaped and not well dispersed. In this work, wedemonstrated that AuNPs of high concentration could be prepared by a two-step approach,i.e. chemical reduction at low concentrations and subsequent centrifugation. Compared tothe direct reduction method, this new two-step method led to AuNPs with high saltresistance and high stability, which are essential for the preparation of DNA-AuNPsconjugates for DNA biodetection.
Keywords: gold nanoparticles; stability; centrifugation; DNA; self-assembly gold nanoparticles; stability; centrifugation; DNA; self-assembly
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liang, Z.; Zhang, J.; Wang, L.; Song, S.; Fan, C.; Li, G. A Centrifugation-based Method for Preparation of Gold Nanoparticles and its Application in Biodetection. Int. J. Mol. Sci. 2007, 8, 526-532.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top