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Int. J. Mol. Sci. 2007, 8(5), 399-406; doi:10.3390/i8050399

Functional Analysis of the Drosophila Dnop5 Using Targeted RNA Interference

Department of Genetics and Developmental Biology, Southeast University Medical School; Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Nanjing 210009, P. R. China
Author to whom correspondence should be addressed.
Received: 7 March 2007 / Revised: 3 May 2007 / Accepted: 9 May 2007 / Published: 31 May 2007
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Dnop5 is a member of the conserved nop5/sik1 gene family, which encodecomponents of small nucleolar ribonucleoprotein(snoRNP) complexes. To study thefunction of DNop5, we generated the polyclonal antibody and determined its expressionpattern. It is highly expressed in different periods of the Drosophila development. We usedheritable RNA interference (RNAi) in combination with the yeast GAL4/UAS binarysystem to knock down the DNop5 protein. It resulted in lethality and dramatic somaticanomalies in RNAi mutant fly, in which the DNop5 protein is reduced efficiently. Northernblotting showed that the processing of 18S rRNA was disrupted in DNop5 knock down fly,but 28S rRNA is normal. These results suggest that DNop5 is essential for the Drosophilagrowth and function in the execution of early pre-rRNA processing steps that lead toformation of 18S rRNA. View Full-Text
Keywords: RNAi; Dnop5; 18S rRNA RNAi; Dnop5; 18S rRNA

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Zhang, Y.; Ding, J.; Wan, Y.; Xie, W.; Yuan, L. Functional Analysis of the Drosophila Dnop5 Using Targeted RNA Interference. Int. J. Mol. Sci. 2007, 8, 399-406.

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