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Int. J. Mol. Sci. 2007, 8(11), 1095-1110; doi:10.3390/i8111092

Antiproliferation of Hepatoma Cell and Progression of Cell Cycle as Affected by Isoflavone Extracts from Soybean Cake

Department of Nutrition and Food Sciences, Fu Jen University, Taipei 242, Taiwan
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Author to whom correspondence should be addressed.
Received: 1 September 2007 / Revised: 1 October 2007 / Accepted: 1 November 2007 / Published: 1 November 2007
(This article belongs to the Special Issue Natural Compounds for Cancer Treatment and Prevention)
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Abstract

The objectives of this study were to isolate various isoflavone fractions andextracts from soybean cake by preparative column chromatography and compare themwith isoflavone standards with regards to inhibition of HepG2 cancer cell proliferation.Four fractions, including malonylglucoside, glucoside, acetylglucoside and aglycone, andtwo isoflavone extracts, ISO-1 and ISO-2, were collected for evaluation. MTT test resultsshowed that most treatments were slightly protective against HepG2 cell growth at a lowdose of isoflavone (5 and 10 μg/mL). However, at elevated concentration of isoflavone(20-50 μg/mL), both aglycone and acetylglucoside fractions as well as a mixture ofisoflavone standards were the most effective in inhibition, demonstrating a possiblesynergistic phenomenon. Genistein showed a better retardation effect than daidzein. Forcell cycle analysis, both aglycone and acetylglucoside fractions and a mixture ofisoflavone standards exhibited a high G2/M ratio, correlating well with the result of MTTtest. The presence of some other functional components in soybean cake like saponinsand phenolic compounds may also play a vital role in inhibiting HepG2 cell growth.
Keywords: Soybean cake; isoflavone extracts; MTT test; cell cycle analysis. Soybean cake; isoflavone extracts; MTT test; cell cycle analysis.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kao, T.-H.; Huang, R.-F.S.; Chen, B.-H. Antiproliferation of Hepatoma Cell and Progression of Cell Cycle as Affected by Isoflavone Extracts from Soybean Cake. Int. J. Mol. Sci. 2007, 8, 1095-1110.

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