Int. J. Mol. Sci. 2006, 7(2), 59-70; doi:10.3390/i7020059
Article

Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line

1 Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, People’s Republic of China 2 Department of General Surgery, Renmin Hospital of Wuhan University Wuhan 430060, People’s Republic of China 3 Zhangzhou Medical and Nursing Vocational School, Zhangzhou, Fujian 363000, People’s Republic of China
* Author to whom correspondence should be addressed.
Received: 11 November 2005; Accepted: 24 January 2006 / Published: 28 February 2006
PDF Full-text Download PDF Full-Text [169 KB, uploaded 20 June 2008 16:51 CEST]
Abstract: The regulation of glucose and sodium butyrate transporters(glucose transporter1-5 and Monocarboxylate transporter 1) and their relationship with cell apoptosis induced bysodium butyrate in colonic caner cell line HT-29 were studied. Cell apoptosis was detectedby flow cytometric assay. The expression of MCT1 and GLUT1-5 mRNA were detected byRT-PCR and the uptake of glucose was detected using 2-deoxy-[3H]glucose. The expressionof bax and bcl-x/l were detected by westernblot assay. We found that sodium butyrateinduced apoptosis in HT-29 cell line. The expression of GLUT1 mRNA, bcl-x/l, as well theuptake of glucose was inhibited by sodium butyrate. The expression of MCT1 and GLUT2,GLUT3, GLUT5 was not regulated by sodium butyrate. However, the concentration ofglucose had positive correlation with the expression of bcl-x/l protein and negativecorrelation with the apoptosis induced by sodium butyrate. All the results suggested thatdownregulation of the expression of GLUT1 was associated with the apoptosis induced bysodium butyrate in HT-29 cell line.
Keywords: Sodium butyrate; Glucose transporter; Monocarboxylate Transporter1; apoptosis; Colon cancer.

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Li, X.; Luo, H.-S.; Paul, S.C.; Tang, T.; Yuan, G.-J. Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line. Int. J. Mol. Sci. 2006, 7, 59-70.

AMA Style

Li X, Luo H-S, Paul SC, Tang T, Yuan G-J. Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line. International Journal of Molecular Sciences. 2006; 7(2):59-70.

Chicago/Turabian Style

Li, Xi; Luo, He-Sheng; Paul, Shelley C.; Tang, Tao; Yuan, Guang-Jin. 2006. "Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line." Int. J. Mol. Sci. 7, no. 2: 59-70.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert