Int. J. Mol. Sci. 2006, 7(2), 59-70; doi:10.3390/i7020059
Article

Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line

1, 1,* email, 1, 2 and 3
Received: 11 November 2005; Accepted: 24 January 2006 / Published: 28 February 2006
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The regulation of glucose and sodium butyrate transporters(glucose transporter1-5 and Monocarboxylate transporter 1) and their relationship with cell apoptosis induced bysodium butyrate in colonic caner cell line HT-29 were studied. Cell apoptosis was detectedby flow cytometric assay. The expression of MCT1 and GLUT1-5 mRNA were detected byRT-PCR and the uptake of glucose was detected using 2-deoxy-[3H]glucose. The expressionof bax and bcl-x/l were detected by westernblot assay. We found that sodium butyrateinduced apoptosis in HT-29 cell line. The expression of GLUT1 mRNA, bcl-x/l, as well theuptake of glucose was inhibited by sodium butyrate. The expression of MCT1 and GLUT2,GLUT3, GLUT5 was not regulated by sodium butyrate. However, the concentration ofglucose had positive correlation with the expression of bcl-x/l protein and negativecorrelation with the apoptosis induced by sodium butyrate. All the results suggested thatdownregulation of the expression of GLUT1 was associated with the apoptosis induced bysodium butyrate in HT-29 cell line.
Keywords: Sodium butyrate; Glucose transporter; Monocarboxylate Transporter1; apoptosis; Colon cancer.
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MDPI and ACS Style

Li, X.; Luo, H.-S.; Paul, S.C.; Tang, T.; Yuan, G.-J. Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line. Int. J. Mol. Sci. 2006, 7, 59-70.

AMA Style

Li X, Luo H-S, Paul SC, Tang T, Yuan G-J. Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line. International Journal of Molecular Sciences. 2006; 7(2):59-70.

Chicago/Turabian Style

Li, Xi; Luo, He-Sheng; Paul, Shelley C.; Tang, Tao; Yuan, Guang-Jin. 2006. "Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line." Int. J. Mol. Sci. 7, no. 2: 59-70.


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