Next Article in Journal / Special Issue
In Vitro Metabolism of Dibenzo[a,l]pyrene, 2-Chlorodibenzo [a,l]pyrene and 10-Chlorodibenzo[a,l]pyrene - Effects of Chloro Substitution
Previous Article in Journal / Special Issue
Effect of Turmerin on Endothelial Denudation by Air Drying
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2002, 3(9), 992-1007; doi:10.3390/i3090992

Transcriptional Activation of Stress Genes and Cytotoxicity in Human Liver Carcinoma (HepG2) Cells Exposed to Pentachlorophenol

Molecular Toxicology Research Laboratory, NIH-Center for Environmental Health, School of Science and Technology, Jackson State University, 1400 Lynch Street, P.O. Box 18540, Jackson, Mississippi 39217, USA
Xenometrix Research Laboratory, Xenometrix, Inc., Boulder, Colorado 80301, USA
Author to whom correspondence should be addressed.
Received: 7 June 2002 / Accepted: 30 September 2002 / Published: 30 September 2002
View Full-Text   |   Download PDF [254 KB, uploaded 19 June 2014]   |  


Pentachlorophenol (PCP) is a biocidal chemical with several industrial, agricultural, and domestic applications. There is accumulating evidence indicating that PCP is highly toxic to humans, with major target organs including the lung, liver, kidneys, heart, and brain. Little is known regarding the molecular basis by which PCP induces toxicity, mutagenesis, and carcinogenesis. Therefore, this research was designed to assess the cellular and molecular responses of HepG2 cells following exposure to PCP. The cytotoxicity experiment yielded a LD50 value of 23.4 + 9.7 μg PCP/mL upon 48 hrs of exposure, indicating that PCP is acutely toxic. A dose-response relationship was recorded with respect to gene induction. For example, fold inductions of CYP1A1 were 1.0 + 0.0, 1.0 + 0.0, 1.3 + 0.5, 6.3 + 4.3, and 22.5 + 3.5 for 0, 6.2, 12.5, 25, and 50 μg PCP/mL, respectively. Overall, five out of the thirteen recombinant cell lines tested showed inductions to statistically significant levels (p < 0.05). At 50 μg PCP/mL, the average fold inductions were 22.5 + 3.5, 52.8 + 2.5, 8.4 + 1.9, 6.16 + 2.4, and 12.5 + 6.8, for CYP1A1, XRE, HMTIIA, c-fos, and GADD153, respectively. These results indicate the potential of PCP to undergo Phase I biotransformation in the liver (CYP1A1, XRE), to cause cell proliferation (c-fos), growth arrest and DNA damage (GADD153), and to influence the toxicokinetics of metal ions (HMTIIA). Marginal inductions were recorded for HSP70, CRE, RARE, GADD45, and GRP78. Within the dose range (0-100 μg/mL) tested, no significant inductions (p < 0.05) were observed for GSTYa, NFkBRE, and p53RE.
Keywords: Pentachlorophenol; cytotoxicity; gene expression; HepG2 cells Pentachlorophenol; cytotoxicity; gene expression; HepG2 cells
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Dorsey, W.C.; Tchounwou, P.B.; Ishaque, A.B.; Shen, E. Transcriptional Activation of Stress Genes and Cytotoxicity in Human Liver Carcinoma (HepG2) Cells Exposed to Pentachlorophenol. Int. J. Mol. Sci. 2002, 3, 992-1007.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top