Surface Biofunctionalization of Gadolinium Phosphate Nanobunches for Boosting Osteogenesis/Chondrogenesis Differentiation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Design and Synthesis of PBLG-g-GdPO4·H2O
2.1.1. Sufficient Suitable Encapsulation of SiO2 onto the Surface of GdPO4·H2O Nanobunches
2.1.2. Further Surface Modification of GdPO4·H2O@SiO2 with Polymer APS and PBLG
2.2. Dispersion and Stability
2.3. T1-Weighted MR Imaging of Modifiers in Biomedical Polymer Material of PLGA
2.4. Cellular Response
2.4.1. Cytotoxicity and Biocompatibility
2.4.2. Osteogenic/Chondrogenic Induction Activity
2.5. Possible Molecular Mechanism
3. Materials and Methods
3.1. Materials
3.2. Preparation of GdPO4·H2O@SiO2
3.3. Synthesis of GdPO4·H2O@SiO2–APS and BLG-NCA
3.4. Synthesis of PBLG-g-GdPO4·H2O
3.5. Preparation of Nanocomposites for MR Imaging and Cell Experiments
3.6. Characterization of Physical and Chemical Properties
3.7. Magnetic Resonance Imaging In Vitro
3.8. In Vitro Cell Culture
3.9. Data Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No. | 1 | 2 | 3 | 4 | 5 |
---|---|---|---|---|---|
mGdPO4·H2O:VTEOS (mg:vL) | 4:5 | 1:1 | 5:4 | 5:3 | 5:2 |
Zeta Potential (mV) | −26.52 | −30.69 | −16.98 | −16.96 | −12.71 |
GdPO4·H2O@SiO2:APS | 1:0.22 | |||
GdPO4·H2O/APS:NCA | 1:0.125 | 1:0.25 | 1:0.5 | 1:1 |
Graft rate of GdPO4·H2O/PBLG (%) | 4.14% | 5.93% | 13.72% | 15.93% |
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Cai, Z.; Guo, Z.; Yang, C.; Wang, F.; Zhang, P.; Wang, Y.; Guo, M.; Wang, Z.; Huang, J.; Zhang, L. Surface Biofunctionalization of Gadolinium Phosphate Nanobunches for Boosting Osteogenesis/Chondrogenesis Differentiation. Int. J. Mol. Sci. 2023, 24, 2032. https://doi.org/10.3390/ijms24032032
Cai Z, Guo Z, Yang C, Wang F, Zhang P, Wang Y, Guo M, Wang Z, Huang J, Zhang L. Surface Biofunctionalization of Gadolinium Phosphate Nanobunches for Boosting Osteogenesis/Chondrogenesis Differentiation. International Journal of Molecular Sciences. 2023; 24(3):2032. https://doi.org/10.3390/ijms24032032
Chicago/Turabian StyleCai, Zhongxing, Ziyi Guo, Chaohui Yang, Fei Wang, Peibiao Zhang, Yu Wang, Min Guo, Zongliang Wang, Jing Huang, and Long Zhang. 2023. "Surface Biofunctionalization of Gadolinium Phosphate Nanobunches for Boosting Osteogenesis/Chondrogenesis Differentiation" International Journal of Molecular Sciences 24, no. 3: 2032. https://doi.org/10.3390/ijms24032032