A Pilot Interaction Analysis of Gut Microbiota and Peripheral Markers of Aging in Severe Psychiatric Disorders: A Role for Lachnoclostridium?
Abstract
:1. Introduction
2. Results
2.1. Characteristics of the Sample
2.2. Interaction Analysis
2.2.1. Interaction Analysis in MDD
2.2.2. Interaction Analysis in SCZ
2.2.3. Adjusting for Possible Bias-Inducing Covariates
3. Discussion
4. Materials and Methods
4.1. Sample
4.2. Sampling Procedures
4.3. Sequencing of Bacterial 16S rRNA Gene
4.4. Peripheral Markers
4.4.1. Quantitative Fluorescence In Situ Hybridization (Q-FISH)
4.4.2. hsCRP
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variable | Level | MDD (N = 31) | SCZ (N = 35) | HCs (N = 15) | p-Value |
---|---|---|---|---|---|
Age (years) | Mean (SD) | 51.9 (12.9) | 46.8 (12.2) | 36.3 (9.1) | <0.001 * |
Sex—N (%) | Males | 9 (29.0) | 31 (88.6) | 10 (66.7) | <0.001 # |
Females | 22 (71.0) | 4 (11.4) | 5 (33.3) | ||
BMI (Kg/m2) | Mean (SD) | 25.2 (5.0) | 27.1 (4.2) | 22.4 (2.2) | <0.001 * |
Family history of mental disorders—N (%) | No | 12 (41.4) | 18 (51.4) | 10 (66.7) | 0.280 # |
Yes | 17 (58.6) | 17 (48.6) | 5 (33.3) | ||
Smoking habits—N (%) | Non-smoker | 16 (51.6) | 8 (22.9) | 11 (73.3) | 0.008 # |
Smoker | 9 (29.0) | 20 (57.1) | 2 (13.3) | ||
Ex-smoker | 6 (19.4) | 7 (20.0) | 2 (13.3) | ||
Drink habits—N (%) ^ | None | 17 (54.8) | 23 (65.8) | 3 (21.4) | 0.137 # |
One occasional drink | 12 (38.7) | 11 (31.6) | 10 (71.4) | ||
1–2 drinks per day | 1 (3.2) | 1 (2.9) | 1 (7.1) | ||
more than 1/2 L per day | 1 (3.2) | 0 (0.0) | 0 (0.0) | ||
Physical activity—N (%) | No | 20 (64.5) | 23 (65.7) | 4 (26.7) | 0.024 # |
Yes | 11 (35.5) | 12 (34.3) | 11 (73.3) | ||
Cardiometabolic comorbidities—N (%) | No | 23 (74.2) | 23 (65.7) | 13 (86.7) | 0.305 # |
Yes | 8 (25.8) | 12 (34.3) | 2 (13.3) | ||
Age at onset (years) | Mean (SD) | 35.5 (7,13) | 25.6 (7.6) | NA | <0.001 * |
Disease duration (years) | Mean (SD) | 16.4 (12.3) | 20.8 (11.9) | NA | 0.144 * |
History of suicide attempts—N (%) | No | 25 (80.6) | 27 (77.1) | NA | 0.77 # |
Yes | 6 (19.4) | 8 (22.9) | NA |
Phylum | Interaction | |||
---|---|---|---|---|
p | OR | CI Low | CI High | |
Actinobacteria | 0.042 * | 1.005 | 1.000 | 1.010 |
Genus | Interaction | |||
p | OR | CI Low | CI High | |
Lachnoclostridium | 0.005 * | 0.981 | 0.968 | 0.994 |
Family | Interaction | |||
---|---|---|---|---|
p | OR | CI Low | CI High | |
Veillonellaceae | 0.02 * | 0.996 | 0.993 | 0.999 |
Genus | Interaction | |||
p | OR | CI Low | CI High | |
Dialister | 0.024 * | 0.996 | 0.992 | 0.999 |
TL | Age | Bacterium | Interaction | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
p | OR | CI Low | CI High | p | OR | CI Low | CI High | p | OR | CI Low | CI High | p | OR | CI Low | CI High |
0.053 | 1.02 | 0.999 | 1.04 | <0.001 * | 1.017 | 1.01 | 1.03 | 0.001 * | 3.28 | 1.36 | 7.92 | 0.001 * | 0.985 | 0.973 | 0.996 |
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Manchia, M.; Paribello, P.; Pisanu, C.; Congiu, D.; Antoniades, A.; Vogazianos, P.; Tozzi, F.; Pinna, F.; Aristodimou, A.; Caria, P.; et al. A Pilot Interaction Analysis of Gut Microbiota and Peripheral Markers of Aging in Severe Psychiatric Disorders: A Role for Lachnoclostridium? Int. J. Mol. Sci. 2023, 24, 17618. https://doi.org/10.3390/ijms242417618
Manchia M, Paribello P, Pisanu C, Congiu D, Antoniades A, Vogazianos P, Tozzi F, Pinna F, Aristodimou A, Caria P, et al. A Pilot Interaction Analysis of Gut Microbiota and Peripheral Markers of Aging in Severe Psychiatric Disorders: A Role for Lachnoclostridium? International Journal of Molecular Sciences. 2023; 24(24):17618. https://doi.org/10.3390/ijms242417618
Chicago/Turabian StyleManchia, Mirko, Pasquale Paribello, Claudia Pisanu, Donatella Congiu, Athos Antoniades, Paris Vogazianos, Federica Tozzi, Federica Pinna, Aristos Aristodimou, Paola Caria, and et al. 2023. "A Pilot Interaction Analysis of Gut Microbiota and Peripheral Markers of Aging in Severe Psychiatric Disorders: A Role for Lachnoclostridium?" International Journal of Molecular Sciences 24, no. 24: 17618. https://doi.org/10.3390/ijms242417618