Oligo-FISH of Populus simonii Pachytene Chromosomes Improves Karyotyping and Genome Assembly
Abstract
:1. Introduction
2. Results
2.1. Karyotyping of Metaphase Chromosomes
2.2. Comparing the Three Methods for Pachytene Chromosomes Preparation
2.3. Karyotyping of Pachytene Chromosomes
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Mitotic Metaphase Chromosome Preparation
4.3. Meiotic Pachytene Chromosome Preparation
4.4. Probes
4.5. Fluorescence In Situ Hybridization
4.6. Cytological Measurements and Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Chr. No. | Long Arm (µm) | Short Arm (µm) | Arm Ratio c | |||
---|---|---|---|---|---|---|
Metaphase a | Pachytene b | Metaphase a | Pachytene b | Metaphase | Pachytene | |
1 | 0.76 ± 0.11 | 31.46 ± 9.21 | 0.52 ± 0.10 | 21.46 ± 6.62 | 1.46 ± 0.17 | 1.48 ± 0.11 |
2 | 0.53 ± 0.08 | 23.69 ± 4.45 | 0.37 ± 0.04 | 6.42 ± 1.17 | 1.46 ± 0.16 | 3.69 ± 0.18 |
3 | 0.56 ± 0.09 | 9.66 ± 0.69 | 0.30 ± 0.03 | 3.01 ± 0.31 | 1.90 ± 0.31 | 3.23 ± 0.28 |
4 | 0.47 ± 0.02 | 10.02 ± 2.260 | 0.33 ± 0.03 | 8.40 ± 1.67 | 1.43 ± 0.16 | 1.19 ± 0.07 |
5 | 0.48 ± 0.05 | 7.62 ± 0.69 | 0.36 ± 0.04 | 5.83 ± 0.45 | 1.35 ± 0.14 | 1.31 ± 0.09 |
6 | 0.48 ± 0.05 | 15.85 ± 2.80 | 0.37 ± 0.05 | 12.27 ± 2.13 | 1.33 ± 0.19 | 1.29 ± 0.07 |
7 | 0.39 ± 0.05 | 4.95 ± 0.44 | 0.30 ± 0.04 | 4.03 ± 0.38 | 1.28 ± 0.09 | 1.23 ± 0.08 |
8 d | 0.55 ± 0.07 | 16.37 ± 2.33 | 0.24 ± 0.04 | 3.33 ± 0.49 | 2.34 ± 0.27 | 4.93 ± 0.32 |
9 | 0.54 ± 0.05 | 9.05 ± 0.94 | 0.18 ± 0.03 | 0.52 ± 0.12 | 3.09 ± 0.59 | 18.21 ± 5.04 |
10 | 0.58 ± 0.09 | 19.53 ± 5.86 | 0.32 ± 0.06 | 3.83 ± 0.81 | 1.85 ± 0.33 | 5.06 ± 0.87 |
11 | 0.53 ± 0.09 | 5.49 ± 0.70 | 0.37 ± 0.04 | 5.00 ± 0.61 | 1.48 ± 0.20 | 1.10 ± 0.04 |
12 | 0.41 ± 0.04 | 4.38 ± 0.50 | 0.31 ± 0.04 | 3.61 ± 0.38 | 1.31 ± 0.14 | 1.21 ± 0.08 |
13 | 0.47 ± 0.03 | 5.06 ± 0.49 | 0.34 ± 0.06 | 3.60 ± 0.42 | 1.41 ± 0.26 | 1.41 ± 0.11 |
14 d | 0.63 ± 0.07 | 15.92 ± 2.00 | 0.23 ± 0.04 | 1.14 ± 0.29 | 2.75 ± 0.45 | 14.81 ± 3.85 |
15 | 0.46 ± 0.03 | 6.57 ± 0.96 | 0.30 ± 0.04 | 4.35 ± 0.67 | 1.54 ± 0.24 | 1.52 ± 0.08 |
16 | 0.49 ± 0.06 | 8.00 ± 1.51 | 0.29 ± 0.05 | 4.70 ± 0.91 | 1.74 ± 0.31 | 1.70 ± 0.06 |
17 | 0.44 ± 0.07 | 4.70 ± 0.39 | 0.31 ± 0.03 | 3.28 ± 0.311 | 1.39 ± 0.20 | 1.43 ± 0.06 |
18 | 0.46 ± 0.04 | 7.76 ± 1.16 | 0.34 ± 0.03 | 5.24 ± 0.74 | 1.39 ± 0.18 | 1.48 ± 0.09 |
19 | 0.42 ± 0.06 | 5.06 ± 0.62 | 0.35 ± 0.05 | 4.27 ± 0.51 | 1.22 ± 0.14 | 1.19 ± 0.06 |
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Zhao, Y.; Liu, G.; Wang, Z.; Ning, Y.; Ni, R.; Xi, M. Oligo-FISH of Populus simonii Pachytene Chromosomes Improves Karyotyping and Genome Assembly. Int. J. Mol. Sci. 2023, 24, 9950. https://doi.org/10.3390/ijms24129950
Zhao Y, Liu G, Wang Z, Ning Y, Ni R, Xi M. Oligo-FISH of Populus simonii Pachytene Chromosomes Improves Karyotyping and Genome Assembly. International Journal of Molecular Sciences. 2023; 24(12):9950. https://doi.org/10.3390/ijms24129950
Chicago/Turabian StyleZhao, Yilian, Guangxin Liu, Ziyue Wang, Yihang Ning, Runxin Ni, and Mengli Xi. 2023. "Oligo-FISH of Populus simonii Pachytene Chromosomes Improves Karyotyping and Genome Assembly" International Journal of Molecular Sciences 24, no. 12: 9950. https://doi.org/10.3390/ijms24129950