Magnesium Nutrient Application Induces Metabolomics and Physiological Responses in Mulberry (Morus alba) Plants
Abstract
:1. Introduction
2. Results
2.1. Plant Biomass Production and Mg Content in Tissues
2.2. Physiological Responses to Mg and Leaf Pigments
2.3. Metabolic Profiles in M. alba Leaves: Responses to Various Applications of Mg
2.4. Analysis of the Differential Metabolites in M. alba Leaves: Responses to Various Mg Applications and Deficiency
2.5. Correlation and Interaction Networks Analysis between the Differential Metabolites’ Profiles
2.6. Metabolic KEGG Pathway and Enrichment Analysis
2.7. Correlation Analysis between the Differential Metabolites’ Profiles and Mulberry Physiological Parameters Measured
3. Discussion
3.1. Physiological Responses to Mg Supply and Deficiency in M. alba
3.2. Differential Metabolites in M. alba Leaves: Response to Various Mg Treatments and Deficiency
3.3. Mulberry Plants’ Response to Mg Supply and Deficiency Induces Amino Acids and Organic Acids
3.4. Lipid Metabolites’ Reponses to Mg Supply and Deficiency in Mulberry Plants
3.5. Mulberry Plants Induce Secondary Metabolites’ Response to Mg Deficiency and Supply
3.6. Correlations between the Differential Metabolite Profiles and Mulberry Physiological Parameters
4. Materials and Methods
4.1. Source of Mulberry Plants and Growth Conditions
4.2. Mineral Analysis
4.3. Physiological Parameters Measurement
4.4. Determination of Chlorophyll Content
4.5. Sample Preparation for Metabolite Extraction
4.6. UHPLC-MS/MS Analysis
4.7. Data Processing and Metabolite Identification
4.8. Data Quality Control
4.9. Hierarchical Cluster Analysis
4.10. PCA and OPLS-DA Analysis
4.11. Differential Metabolite Identification and KEGG Annotations and Enrichment Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jin, X.; Ackah, M.; Wang, L.; Amoako, F.K.; Shi, Y.; Essoh, L.G.; Li, J.; Zhang, Q.; Li, H.; Zhao, W. Magnesium Nutrient Application Induces Metabolomics and Physiological Responses in Mulberry (Morus alba) Plants. Int. J. Mol. Sci. 2023, 24, 9650. https://doi.org/10.3390/ijms24119650
Jin X, Ackah M, Wang L, Amoako FK, Shi Y, Essoh LG, Li J, Zhang Q, Li H, Zhao W. Magnesium Nutrient Application Induces Metabolomics and Physiological Responses in Mulberry (Morus alba) Plants. International Journal of Molecular Sciences. 2023; 24(11):9650. https://doi.org/10.3390/ijms24119650
Chicago/Turabian StyleJin, Xin, Michael Ackah, Lei Wang, Frank Kwarteng Amoako, Yisu Shi, Lionnelle Gyllye Essoh, Jianbin Li, Qiaonan Zhang, Haonan Li, and Weiguo Zhao. 2023. "Magnesium Nutrient Application Induces Metabolomics and Physiological Responses in Mulberry (Morus alba) Plants" International Journal of Molecular Sciences 24, no. 11: 9650. https://doi.org/10.3390/ijms24119650