Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice
Abstract
:1. Introduction
2. Results
2.1. Bone Marrow Cell Characterisation
2.2. Bone Marrow-Derived Dendritic Cell Activation
2.3. Breast Cancer and Colorectal Cancer Tumour Growth Rate in Lean and Obese Mice
2.4. Tumour-Infiltrating Lymphocytes Phenotyping
2.5. Frequency of Tumour-Infiltrating Lymphocytes
2.6. Peripheral Blood Populations
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Phenotyping of Bone Marrow Cells
4.3. Generation and Activation of BMDCs
4.4. Tumour Cell Lines and Tumour Challenges
4.5. Assessment of Tumour-Infiltrating Lymphocytes
4.6. Phenotyping of Peripheral Blood Immune Cell Populations
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Neumann, S.; Campbell, K.; Woodall, M.J.; Evans, M.; Clarkson, A.N.; Young, S.L. Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice. Int. J. Mol. Sci. 2021, 22, 8803. https://doi.org/10.3390/ijms22168803
Neumann S, Campbell K, Woodall MJ, Evans M, Clarkson AN, Young SL. Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice. International Journal of Molecular Sciences. 2021; 22(16):8803. https://doi.org/10.3390/ijms22168803
Chicago/Turabian StyleNeumann, Silke, Katrin Campbell, Matthew J. Woodall, Meghan Evans, Andrew N. Clarkson, and Sarah L. Young. 2021. "Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice" International Journal of Molecular Sciences 22, no. 16: 8803. https://doi.org/10.3390/ijms22168803