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Int. J. Mol. Sci. 2018, 19(9), 2613; https://doi.org/10.3390/ijms19092613

Evaluation of Toxicity and Neural Uptake In Vitro and In Vivo of Superparamagnetic Iron Oxide Nanoparticles

1
Institute of Process Engineering, Chair of Mechanical Process Engineering, Otto-von-Guericke University, Universitätsplatz 2, 39106 Magdeburg, Germany
2
Institute of Medical Psychology, Otto-von-Guericke University, Leipziger Str. 44, 39120 Magdeburg, Germany
3
German Center for Neurodegenerative Diseases, Leipziger Str. 44, 39120 Magdeburg, Germany
4
Medical Faculty, Otto-von-Guericke University, Leipziger Str. 44, 39120 Magdeburg, Germany
5
Center for Behavioral Brain Sciences (CBBS), 39106 Magdeburg, Germany
*
Author to whom correspondence should be addressed.
Received: 24 August 2018 / Revised: 31 August 2018 / Accepted: 31 August 2018 / Published: 3 September 2018
(This article belongs to the Section Molecular Toxicology)
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Abstract

Superparamagnetic iron oxide nanoparticles (SPIO-NPs) have great potential to be used in different pharmaceutical applications, due to their unique and versatile physical and chemical properties. The aim of this study was to quantify in vitro cytotoxicity of dextran 70,000-coated SPIO-NPs labelled/unlabelled with rhodamine 123, in C6 glioma cells and primary hippocampal neural cells. In addition, we analyzed the in vitro and in vivo cellular uptake of labelled SPIO-NPs. The nanoparticles, with average size of 10–50 nm and polydispersity index of 0.37, were synthesized using Massart’s co-precipitation method. The concentration-dependent cytotoxicity was quantified by using tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Intracellular localization of SPIO-NPs was detected by confocal laser microscopy. In vivo confocal neuroimaging (ICON) was performed on male Wistar rats after intravitreal injection followed by ex vivo retina whole mount analysis. When used for in vitro testing concentrations in the range of diagnostic and therapeutic dosages, SPIO-NPs proved to be non-cytotoxic on C6 glioma cells for up to 24 h incubation time. The hippocampal cell culture also did not show impaired viability at low doses after 24 h incubation. Our results indicate that our dextran-coated SPIO-NPs have the potential for in vivo drug delivery applications. View Full-Text
Keywords: superparamagnetic iron oxide nanoparticles (SPIO-NPs); drug delivery; in vitro toxicity; cellular uptake superparamagnetic iron oxide nanoparticles (SPIO-NPs); drug delivery; in vitro toxicity; cellular uptake
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Khalid, M.K.; Asad, M.; Henrich-Noack, P.; Sokolov, M.; Hintz, W.; Grigartzik, L.; Zhang, E.; Dityatev, A.; van Wachem, B.; Sabel, B.A. Evaluation of Toxicity and Neural Uptake In Vitro and In Vivo of Superparamagnetic Iron Oxide Nanoparticles. Int. J. Mol. Sci. 2018, 19, 2613.

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