Evaluation of Active Brown Adipose Tissue by the Use of Hyperpolarized [1-13C]Pyruvate MRI in Mice
AbstractThe capacity to increase energy expenditure makes brown adipose tissue (BAT) a putative target for treatment of metabolic diseases such as obesity. Presently, investigation of BAT in vivo is mainly performed by fluoro-d-glucose positron emission tomography (FDG PET)/CT. However, non-radioactive methods that add information on, for example, substrate metabolism are warranted. Thus, the aim of this study was to evaluate the potential of hyperpolarized [1-13C]pyruvate Magnetic Resonance Imaging (HP-MRI) to determine BAT activity in mice following chronic cold exposure. Cold (6 °C) and thermo-neutral (30 °C) acclimated mice were scanned with HP-MRI for assessment of the interscapular BAT (iBAT) activity. Comparable mice were scanned with the conventional method FDG PET/MRI. Finally, iBAT was evaluated for gene expression and protein levels of the specific thermogenic marker, uncoupling protein 1 (UCP1). Cold exposure increased the thermogenic capacity 3–4 fold (p < 0.05) as measured by UCP1 gene and protein analysis. Furthermore, cold exposure as compared with thermo-neutrality increased iBAT pyruvate metabolism by 5.5-fold determined by HP-MRI which is in good agreement with the 5-fold increment in FDG uptake (p < 0.05) measured by FDG PET/MRI. iBAT activity is detectable in mice using HP-MRI in which potential changes in intracellular metabolism may add useful information to the conventional FDG PET studies. HP-MRI may also be a promising radiation-free tool for repetitive BAT studies in humans. View Full-Text
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Riis-Vestergaard, M.J.; Breining, P.; Pedersen, S.B.; Laustsen, C.; Stødkilde-Jørgensen, H.; Borghammer, P.; Jessen, N.; Richelsen, B. Evaluation of Active Brown Adipose Tissue by the Use of Hyperpolarized [1-13C]Pyruvate MRI in Mice. Int. J. Mol. Sci. 2018, 19, 2597.
Riis-Vestergaard MJ, Breining P, Pedersen SB, Laustsen C, Stødkilde-Jørgensen H, Borghammer P, Jessen N, Richelsen B. Evaluation of Active Brown Adipose Tissue by the Use of Hyperpolarized [1-13C]Pyruvate MRI in Mice. International Journal of Molecular Sciences. 2018; 19(9):2597.Chicago/Turabian Style
Riis-Vestergaard, Mette J.; Breining, Peter; Pedersen, Steen B.; Laustsen, Christoffer; Stødkilde-Jørgensen, Hans; Borghammer, Per; Jessen, Niels; Richelsen, Bjørn. 2018. "Evaluation of Active Brown Adipose Tissue by the Use of Hyperpolarized [1-13C]Pyruvate MRI in Mice." Int. J. Mol. Sci. 19, no. 9: 2597.
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