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Int. J. Mol. Sci. 2018, 19(7), 2055; https://doi.org/10.3390/ijms19072055

Poly Organotin Acetates against DNA with Possible Implementation on Human Breast Cancer

1
Section of Inorganic and Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece
2
Medical Physics Laboratory, Medical School, University of Ioannina, 45110 Ioannina, Greece
3
Department of Chemistry, University of Cyprus, 1678 Nicosia, Cyprus
4
Mössbauer Spectroscopy and Physics of Material Laboratory, Department of Physics, University of Ioannina, 45110 Ioannina, Greece
5
Physical Chemistry Laboratory, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece
*
Authors to whom correspondence should be addressed.
Received: 16 June 2018 / Revised: 6 July 2018 / Accepted: 12 July 2018 / Published: 14 July 2018
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Abstract

Two known tin-based polymers of formula {[R3Sn(CH3COO)]n} where R = n-Bu– (1) and R = Ph– (2),were evaluated for their in vitro biological properties. The compounds were characterized via their physical properties and FT-IR, 119Sn Mössbauer, and 1H NMR spectroscopic data. The molecular structures were confirmed by single-crystal X-Ray diffraction crystallography. The geometry around the tin(IV) ion is trigonal bi-pyramidal. Variations in O–Sn–O···Sn′ torsion angles lead to zig-zag and helical supramolecular assemblies for 1 and 2, respectively. The in vitro cell viability against human breast adenocarcinoma cancer cell lines: MCF-7 positive to estrogens receptors (ERs) and MDA-MB-231 negative to ERs upon their incubation with 1 and 2 was investigated. Their toxicity has been studied against normal human fetal lung fibroblast cells (MRC-5). Compounds 1 and 2 exhibit 134 and 223-fold respectively stronger antiproliferative activity against MDA-MB-231 than cisplatin. The type of the cell death caused by 1 or 2 was also determined using flow cytometry assay. The binding affinity of 1 and 2 towards the CT-DNA was suspected from the differentiation of the viscosity which occurred in the solution containing increasing amounts of 1 and 2. Changes in fluorescent emission light of Ethidium bromide (EB) in the presence of DNA confirmed the intercalation mode of interactions into DNA of both complexes 1 and 2 which have been ascertained from viscosity measurements. The corresponding apparent binding constants (Kapp) of 1 and 2 towards CT-DNA calculated through fluorescence spectra are 4.9 × 104 (1) and 7.3 × 104 (2) M−1 respectively. Finally, the type of DNA binding interactions with 1 and 2 was confirmed by docking studies. View Full-Text
Keywords: biological inorganic chemistry; acetic acid; organotins; bio-polymer; anti-cancer activity; cell cycle biological inorganic chemistry; acetic acid; organotins; bio-polymer; anti-cancer activity; cell cycle
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Latsis, G.K.; Banti, C.N.; Kourkoumelis, N.; Papatriantafyllopoulou, C.; Panagiotou, N.; Tasiopoulos, A.; Douvalis, A.; Kalampounias, A.G.; Bakas, T.; Hadjikakou, S.K. Poly Organotin Acetates against DNA with Possible Implementation on Human Breast Cancer. Int. J. Mol. Sci. 2018, 19, 2055.

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