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Int. J. Mol. Sci. 2018, 19(5), 1464; https://doi.org/10.3390/ijms19051464

Inhibition of Proliferation in U937 Cells Treated by Blue Light Irradiation and Combined Blue Light Irradiation/Drug

State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, China
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Received: 19 March 2018 / Revised: 9 May 2018 / Accepted: 9 May 2018 / Published: 15 May 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The cell viability and apoptosis of tumor U937 cells treated by blue light (BL) irradiation have been examined. BL irradiation can specially inhibit the proliferation and promote the apoptosis of U937 cells, relating to the production of reactive oxygen species (ROS) and the decline of mitochondrial membrane potential (ΔΨm). The apoptosis is further associated with varying downregulated B-cell lymphoma-extra large (Bcl-XL) and B-cell lymphoma 2 (Bcl-2) genes, upregulated Bcl-2-associated X (Bax) gene, the activation of caspase-3 and caspase-9, and the cleavage of poly (ADP-ribose) polymerase (PARP) by the BL irradiation process. Moreover, BL irradiation induced proliferation inhibition is higher than that treated by a common chemotherapeutic drug of homoharringtonine (HHT). When we synergize BL irradiation with HHT (BL-HHT), a higher proliferation inhibition is obtained than that treated by BL irradiation or HHT alone. These results are helpful for establishing a low toxicity and high efficiency strategy of BL irradiation for clinical treatment of acute myeloid leukemia, not limited to U937 cells. View Full-Text
Keywords: enhanced proliferation inhibition; U937 cells; BL irradiation; apoptosis enhanced proliferation inhibition; U937 cells; BL irradiation; apoptosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhuang, J.; Liu, J.; Gao, X.; Li, H. Inhibition of Proliferation in U937 Cells Treated by Blue Light Irradiation and Combined Blue Light Irradiation/Drug. Int. J. Mol. Sci. 2018, 19, 1464.

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