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Int. J. Mol. Sci. 2018, 19(4), 1201; https://doi.org/10.3390/ijms19041201

Serum Is Not Necessary for Prior Pharmacological Activation of AMPK to Increase Insulin Sensitivity of Mouse Skeletal Muscle

Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, DK-2100 Copenhagen, Denmark
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Received: 23 March 2018 / Revised: 9 April 2018 / Accepted: 10 April 2018 / Published: 15 April 2018
(This article belongs to the Special Issue AMP-Activated Protein Kinase Signalling)
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Abstract

Exercise, contraction, and pharmacological activation of AMP-activated protein kinase (AMPK) by 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) have all been shown to increase muscle insulin sensitivity for glucose uptake. Intriguingly, improvements in insulin sensitivity following contraction of isolated rat and mouse skeletal muscle and prior AICAR stimulation of isolated rat skeletal muscle seem to depend on an unknown factor present in serum. One study recently questioned this requirement of a serum factor by showing serum-independency with muscle from old rats. Whether a serum factor is necessary for prior AICAR stimulation to increase insulin sensitivity of mouse skeletal muscle is not known. Therefore, we investigated the necessity of serum for this effect of AICAR in mouse skeletal muscle. We found that the ability of prior AICAR stimulation to improve insulin sensitivity of mouse skeletal muscle did not depend on the presence of serum during AICAR stimulation. Although prior AICAR stimulation did not enhance proximal insulin signaling, insulin-stimulated phosphorylation of Tre-2/BUB2/CDC16- domain family member 4 (TBC1D4) Ser711 was greater in prior AICAR-stimulated muscle compared to all other groups. These results imply that the presence of a serum factor is not necessary for prior AMPK activation by AICAR to enhance insulin sensitivity of mouse skeletal muscle. View Full-Text
Keywords: exercise; glucose uptake; AMP-activated protein kinase; TBC1D4; AS160 exercise; glucose uptake; AMP-activated protein kinase; TBC1D4; AS160
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Jørgensen, N.O.; Wojtaszewski, J.F.P.; Kjøbsted, R. Serum Is Not Necessary for Prior Pharmacological Activation of AMPK to Increase Insulin Sensitivity of Mouse Skeletal Muscle. Int. J. Mol. Sci. 2018, 19, 1201.

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