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Int. J. Mol. Sci. 2018, 19(4), 1164; doi:10.3390/ijms19041164

A Novel Synthetic Material, BMM, Accelerates Wound Repair by Stimulating Re-Epithelialization and Fibroblast Activation

1
Department of Biochemistry, School of Medicine, Jeju National University, Jeju 690-756, Korea
2
Department of Pathology, School of Medicine, Jeju National University, Jeju 690-756, Korea
3
Department of Applied Chemistry, Dongduk Women’s University, Seoul 136-714, Korea
4
Division of Life & Environmental Science, Daegu University, Gyeongsangbuk-do 38453, Korea
5
Division of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
6
Institute of Medical Science, Jeju National University, Jeju 690-756, Korea
*
Authors to whom correspondence should be addressed.
Received: 9 March 2018 / Revised: 3 April 2018 / Accepted: 9 April 2018 / Published: 11 April 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Cutaneous wound repair is an intricate process whereby the skin reprograms itself after injury. In the mid-phase of wound repair, the proliferation, migration, and differentiation of cells are the major mechanisms to lead remodeling. We investigated the effect of BMM ((1E,2E)-1,2-bis((6-bromo-2H-chromen-3-yl)methylene)hydrazine), a novel synthetic material, on the migration and viability of keratinocytes or fibroblasts using the in vitro scratch woundhealing, electric cell-substrate imedance sensing (ECIS), invasion, and MTT assays. Cell migration-related factors were analyzed using western blot, and we found that treatment with BMM stimulated the EMT pathway and focal adhesion kinase (FAK)/Src signaling. Differentiation of HaCaT keratinocyte and fibroblast cells was also stimulated by BMM and specifically, NOX2/4 contributed to the activation of fibroblasts for wound healing. Furthermore, BMM treated HaCaT keratinocyte and fibroblast-co-cultured cells increased migration and differentiation. TGF-β and Cyr61 were also secreted to a greater extent than in single cultured cells. In vivo experiments showed that treatment with BMM promotes wound closure by promoting re-epithelialization. In this study, we demonstrated that a novel synthetic material, BMM, is capable of promoting wound healing via the stimulation of re-epithelialization in the epidermis and the activation of fibroblasts in the dermis, in particular, via the acceleration of the interaction between the epidermis and dermis. View Full-Text
Keywords: wound healing; re-epithelialization; fibroplasia; TGF-β; Cyr61; NADPH oxidase wound healing; re-epithelialization; fibroplasia; TGF-β; Cyr61; NADPH oxidase
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Seo, G.Y.; Hyun, C.; Koh, D.; Park, S.; Lim, Y.; Kim, Y.M.; Cho, M. A Novel Synthetic Material, BMM, Accelerates Wound Repair by Stimulating Re-Epithelialization and Fibroblast Activation. Int. J. Mol. Sci. 2018, 19, 1164.

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