Integrins Were Involved in Soybean Agglutinin Induced Cell Apoptosis in IPEC-J2
AbstractAbstract: Soybean agglutinin (SBA), is a non-fiber carbohydrate related protein and a major anti-nutritional factor. Integrins, transmembrane glycoproteins, are involved in many biological processes. Although recent work suggested that integrins are involved in SBA-induced cell-cycle alterations, no comprehensive study has reported whether integrins are involved in SBA-induced cell apoptosis (SCA) in IPEC-J2. The relationship between SBA and integrins are still unclear. We aimed to elucidate the effects of SBA on IPEC-J2 cell proliferation and cell apoptosis; to study the roles of integrins in IPEC-J2 normal cell apoptosis (NCA) and SCA; and to illustrate the relationship and connection type between SBA and integrins. Thus, IPEC-J2 cells were treated with SBA at the levels of 0, 0.125, 0.25, 0.5, 1.0 or 2.0 mg/mL to determine cell proliferation and cell apoptosis. The cells were divided into control, SBA treated groups, integrin inhibitor groups, and SBA + integrin inhibitor groups to determine the integrin function in SCA. The results showed that SBA significantly (p < 0.05) lowered cell proliferation and induced cell apoptosis in IPEC-J2 (p < 0.05). Inhibition of any integrin type induced the cell apoptosis (p < 0.05) and these integrins were involved in SCA (p < 0.05). Even SBA had no physical connection with integrins, an association was detected between SBA and α-actinin-2 ACTN2 (integrin-binding protein). Additionally, SBA reduced the mRNA expression of integrins by down regulating the gene expression level of ACTN2. We concluded an evidence for the anti-nutritional mechanism of SBA by ACTN2 with integrins. Further trials are needed to prove whether ACTN2 is the only protein for connecting SBA with integrin. View Full-Text
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Pan, L.; Zhao, Y.; Farouk, M.H.; Bao, N.; Wang, T.; Qin, G. Integrins Were Involved in Soybean Agglutinin Induced Cell Apoptosis in IPEC-J2. Int. J. Mol. Sci. 2018, 19, 587.
Pan L, Zhao Y, Farouk MH, Bao N, Wang T, Qin G. Integrins Were Involved in Soybean Agglutinin Induced Cell Apoptosis in IPEC-J2. International Journal of Molecular Sciences. 2018; 19(2):587.Chicago/Turabian Style
Pan, Li; Zhao, Yuan; Farouk, Mohammed Hamdy; Bao, Nan; Wang, Tao; Qin, Guixin. 2018. "Integrins Were Involved in Soybean Agglutinin Induced Cell Apoptosis in IPEC-J2." Int. J. Mol. Sci. 19, no. 2: 587.
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