Comparative Membrane-Associated Proteomics of Three Different Immune Reactions in Potato
AbstractPlants have evolved different types of immune reactions but large-scale proteomics about these processes are lacking, especially in the case of agriculturally important crop pathosystems. We have established a system for investigating PAMP-triggered immunity (PTI) and two different effector-triggered immunity (ETI; triggered by Avr2 or IpiO) responses in potato. The ETI responses are triggered by molecules from the agriculturally important Phytophthora infestans interaction. To perform large-scale membrane protein-based comparison of these responses, we established a method to extract proteins from subcellular compartments in leaves. In the membrane fractions that were subjected to quantitative proteomics analysis, we found that most proteins regulated during PTI were also regulated in the same way in ETI. Proteins related to photosynthesis had lower abundance, while proteins related to oxidative and biotic stress, as well as those related to general antimicrobial defense and cell wall degradation, were found to be higher in abundance. On the other hand, we identified a few proteins—for instance, an ABC transporter-like protein—that were only found in the PTI reaction. Furthermore, we also identified proteins that were regulated only in ETI interactions. These included proteins related to GTP binding and heterotrimeric G-protein signaling, as well as those related to phospholipase signaling. View Full-Text
- Supplementary File 1:
XLSX-Document (XLSX, 647 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Burra, D.D.; Lenman, M.; Levander, F.; Resjö, S.; Andreasson, E. Comparative Membrane-Associated Proteomics of Three Different Immune Reactions in Potato. Int. J. Mol. Sci. 2018, 19, 538.
Burra DD, Lenman M, Levander F, Resjö S, Andreasson E. Comparative Membrane-Associated Proteomics of Three Different Immune Reactions in Potato. International Journal of Molecular Sciences. 2018; 19(2):538.Chicago/Turabian Style
Burra, Dharani D.; Lenman, Marit; Levander, Fredrik; Resjö, Svante; Andreasson, Erik. 2018. "Comparative Membrane-Associated Proteomics of Three Different Immune Reactions in Potato." Int. J. Mol. Sci. 19, no. 2: 538.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.