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Int. J. Mol. Sci. 2018, 19(2), 471; https://doi.org/10.3390/ijms19020471

Importance of a Laccase Gene (Lcc1) in the Development of Ganoderma tsugae

1
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Gutian 352200, China
3
Institute of Occean Science, Minjiang University, Fuzhou 350002, China
*
Authors to whom correspondence should be addressed.
Received: 31 December 2017 / Revised: 28 January 2018 / Accepted: 1 February 2018 / Published: 6 February 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

In this study, a novel laccase gene (Lcc1) from Ganoderma tsugae was isolated and its functions were characterized in detail. The results showed that Lcc1 has the highest expression activity during mycelium development and fruit body maturation based on the analysis of Lcc1 RNA transcripts at different developmental stages of G. tsugae. To investigate the exact contribution of Lcc1 to mycelium and fruit body development in G. tsugae, Lcc1 transgenic strains were constructed by targeted gene replacement and over-expression approaches. The results showed that the lignin degradation rate in Lcc1 deletion mutant was much lower than the degradation efficiency of the wild-type (WT), over-expression and rescue strains. The lignin degradation activity of G. tsugae is dependent on Lcc1 and the deletion of Lcc1 exerted detrimental influences on the development of mycelium branch. Furthermore, the study uncovered that Lcc1 deletion mutants generated much shorter pale grey fruit bodies, suggesting that Lcc1 contributes directly to pigmentation and stipe elongation during fruit body development in G. tsugae. The information obtained in this study provides a novel and mechanistic insight into the specific role of Lcc1 during growth and development of G. tsugae. View Full-Text
Keywords: Ganoderma tsugae; laccase; targeted gene replacement; over-expression; development Ganoderma tsugae; laccase; targeted gene replacement; over-expression; development
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jin, W.; Li, J.; Feng, H.; You, S.; Zhang, L.; Norvienyeku, J.; Hu, K.; Sun, S.; Wang, Z. Importance of a Laccase Gene (Lcc1) in the Development of Ganoderma tsugae. Int. J. Mol. Sci. 2018, 19, 471.

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