VEGF Triggers the Activation of Cofilin and the Arp2/3 Complex within the Growth Cone
Abstract
:1. Introduction
2. Results
2.1. Analysis of the Distribution of Cofilin and Actin in the Growth Cone
2.2. VEGF Leads to Rapid Changes in Cofilin Distribution and Repels Growth Cones When LIMK is Inhibited
2.3. Analysis of the Distribution of the Arp 2/3-Complex within the Axonal Growth Cone
2.4. VEGF Triggers the Redistribution of Arp2/3 and Repels Growth Cones When N-WASP is Inhibited
3. Discussion
3.1. VEGF Triggers a Change in the Distribution of Cofilin in a LIMK-Dependent Pathway
3.2. VEGF Triggers the Distribution of the Arp2/3 Complex within the Growth Cone in an N-WASP Dependent Pathway
4. Materials and Methods
4.1. Immunohistochemistry and Evaluation of Morphology
4.1.1. DRG Explants
4.1.2. Immunohistochemistry
4.1.3. Analysis of Growth Cone Morphology
4.2. Live Cell Imaging
4.2.1. Dissociated DRG Cell Cultures
4.2.2. Microinjection
4.2.3. Live Cell Imaging with the Confocal Laser Scanning Microscope
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Schlau, M.; Terheyden-Keighley, D.; Theis, V.; Mannherz, H.G.; Theiss, C. VEGF Triggers the Activation of Cofilin and the Arp2/3 Complex within the Growth Cone. Int. J. Mol. Sci. 2018, 19, 384. https://doi.org/10.3390/ijms19020384
Schlau M, Terheyden-Keighley D, Theis V, Mannherz HG, Theiss C. VEGF Triggers the Activation of Cofilin and the Arp2/3 Complex within the Growth Cone. International Journal of Molecular Sciences. 2018; 19(2):384. https://doi.org/10.3390/ijms19020384
Chicago/Turabian StyleSchlau, Matthias, Daniel Terheyden-Keighley, Verena Theis, Hans Georg Mannherz, and Carsten Theiss. 2018. "VEGF Triggers the Activation of Cofilin and the Arp2/3 Complex within the Growth Cone" International Journal of Molecular Sciences 19, no. 2: 384. https://doi.org/10.3390/ijms19020384