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Int. J. Mol. Sci. 2018, 19(2), 332; doi:10.3390/ijms19020332

Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye

1
Marian Smoluchowski Institute of Physics, Jagiellonian University, Prof. Stanisława Łojasiewicza 11 Street, 30-348 Krakow, Poland
2
Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7 Street, 30-387 Krakow, Poland
3
Ophthalmology and Ocular Oncology Clinic, University Hospital, Kopernika 38 Street, 31-501 Krakow, Poland
*
Author to whom correspondence should be addressed.
Received: 31 December 2017 / Revised: 16 January 2018 / Accepted: 18 January 2018 / Published: 24 January 2018
(This article belongs to the Special Issue Animal Models of Melanoma)
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Abstract

A tumor vasculature network undergoes intense growth and rebuilding during tumor growth. Traditionally, vascular networks are histologically examined using parameters such as vessel density determined from two-dimensional slices of the tumor. Two-dimensional probing of a complicated three-dimensional (3D) structure only provides partial information. Therefore, we propose the use of microcomputed tomography (micro-CT) imaging to analyze the evolution of a tumor vasculature in an experimental ocular tumor model. A Bomirski Hamster Melanoma was implanted in the anterior chamber of a hamster eye. Ultrasound (US) imaging of the same tumor was performed in vivo, and the vascular results obtained using the two methods were compared. Normal ocular tissues, a tumor, and a tumor vascular structure were revealed with high accuracy using micro-CT. The vessels that grew within the tumor were chaotic, leaky, and contained many convoluted micro-vessels and embolizations. They comprised 20–38% of the tumor mass. The blood flow in the larger functional vessels was in the range from 10 to 25 mm/s, as determined by in vivo Doppler US. The micro-CT imaging of the hamster eyeball enabled both qualitative and quantitative 3D analyses of the globe at a histological level. Although the presented images were obtained ex vivo, micro-CT noninvasive imaging is being developed intensively, and high-resolution in vivo imaging is feasible. View Full-Text
Keywords: imaging; tumor vasculature; ocular tumors; melanoma; hamster; micro-CT; ultrasound imaging; tumor vasculature; ocular tumors; melanoma; hamster; micro-CT; ultrasound
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Leszczyński, B.; Śniegocka, M.; Wróbel, A.; Pędrys, R.; Szczygieł, M.; Romanowska-Dixon, B.; Urbańska, K.; Elas, M. Visualization and Quantitative 3D Analysis of Intraocular Melanoma and Its Vascularization in a Hamster Eye. Int. J. Mol. Sci. 2018, 19, 332.

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