Next Article in Journal
Key Clinical Factors Predicting Adipokine and Oxidative Stress Marker Concentrations among Normal, Overweight and Obese Pregnant Women Using Artificial Neural Networks
Next Article in Special Issue
Genome-Wide Identification and Characterization of WD40 Protein Genes in the Silkworm, Bombyx mori
Previous Article in Journal
Identification of Host Defense-Related Proteins Using Label-Free Quantitative Proteomic Analysis of Milk Whey from Cows with Staphylococcus aureus Subclinical Mastitis
Previous Article in Special Issue
Identification of Differentially Expressed miRNAs in Colorado Potato Beetles (Leptinotarsa decemlineata (Say)) Exposed to Imidacloprid
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(1), 83; https://doi.org/10.3390/ijms19010083

New Method for Differentiation of Granuloviruses (Betabaculoviruses) Based on Multitemperature Single Stranded Conformational Polymorphism

1
Department of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, 80-807 Gdansk, Poland
2
Embrapa Recursos Genéticos e Biotecnologia, Parque Estacao Biológica, 70770-900 Brasilia, Brazil
3
Citrus Research International (CRI), P.O. Box 20285, Humewood 6013, Port Elizabeth, South Africa
4
Department of Zoology and Entomology, Rhodes University, P.O. Box 94, Grahamstown 6140, South Africa
*
Author to whom correspondence should be addressed.
Received: 15 November 2017 / Revised: 20 December 2017 / Accepted: 23 December 2017 / Published: 28 December 2017
(This article belongs to the Special Issue Molecular Entomology of Insects of Economic Importance)
View Full-Text   |   Download PDF [2620 KB, uploaded 28 December 2017]   |  

Abstract

Baculoviruses have been used as biopesticides for decades. Recently, due to the excessive use of chemical pesticides there is a need for finding new agents that may be useful in biological protection. Sometimes few isolates or species are discovered in one host. In the past few years, many new baculovirus species have been isolated from environmental samples, thoroughly characterized and thanks to next generation sequencing methods their genomes are being deposited in the GenBank database. Next generation sequencing (NGS) methodology is the most certain way of detection, but it has many disadvantages. During our studies, we have developed a method based on Polymerase chain reaction (PCR) followed by Multitemperature Single Stranded Conformational Polymorphism (MSSCP) which allows for distinguishing new granulovirus isolates in only a few hours and at low-cost. On the basis of phylogenetic analysis of betabaculoviruses, representative species have been chosen. The alignment of highly conserved genes—granulin and late expression factor-9, was performed and the degenerate primers were designed to amplify the most variable, short DNA fragments flanked with the most conserved sequences. Afterwards, products of PCR reaction were analysed by MSSCP technique. In our opinion, the proposed method may be used for screening of new isolates derived from environmental samples. View Full-Text
Keywords: baculovirus detection; granulovirus detection; betabaculovirus; MSSCP; PCR; granulin; late expression factor-9 baculovirus detection; granulovirus detection; betabaculovirus; MSSCP; PCR; granulin; late expression factor-9
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Krejmer-Rabalska, M.; Rabalski, L.; Lobo de Souza, M.; Moore, S.D.; Szewczyk, B. New Method for Differentiation of Granuloviruses (Betabaculoviruses) Based on Multitemperature Single Stranded Conformational Polymorphism. Int. J. Mol. Sci. 2018, 19, 83.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top