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Int. J. Mol. Sci. 2017, 18(12), 2728; doi:10.3390/ijms18122728

Identification of Differentially Expressed miRNAs in Colorado Potato Beetles (Leptinotarsa decemlineata (Say)) Exposed to Imidacloprid

1
Department of Chemistry and Biochemistry, University of Moncton, 18 Antonine-Maillet Avenue, Moncton, NB E1A 3E9, Canada
2
Atlantic Cancer Research Institute, Pavillon Hôtel-Dieu 35 Providence Street, Moncton, NB E1C 8X3, Canada
3
Fredericton Research and Development Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, Fredericton, NB E3B 4Z7, Canada
*
Author to whom correspondence should be addressed.
Received: 3 November 2017 / Revised: 8 December 2017 / Accepted: 12 December 2017 / Published: 16 December 2017
(This article belongs to the Special Issue Molecular Entomology of Insects of Economic Importance)
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Abstract

The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is a significant pest of potato plants that has been controlled for more than two decades by neonicotinoid imidacloprid. L. decemlineata can develop resistance to this agent even though the molecular mechanisms underlying this resistance are not well characterized. MicroRNAs (miRNAs) are short ribonucleic acids that have been linked to response to various insecticides in several insect models. Unfortunately, the information is lacking regarding differentially expressed miRNAs following imidacloprid treatment in L. decemlineata. In this study, next-generation sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) were used to identify modulated miRNAs in imidacloprid-treated versus untreated L. decemlineata. This approach identified 33 differentially expressed miRNAs between the two experimental conditions. Of interest, miR-282 and miR-989, miRNAs previously shown to be modulated by imidacloprid in other insects, and miR-100, a miRNA associated with regulation of cytochrome P450 expression, were significantly modulated in imidacloprid-treated beetles. Overall, this work presents the first report of a miRNA signature associated with imidacloprid exposure in L. decemlineata using a high-throughput approach. It also reveals interesting miRNA candidates that potentially underly imidacloprid response in this insect pest. View Full-Text
Keywords: microRNAs; cold hardiness; Colorado potato beetles; imidacloprid; next-generation sequencing microRNAs; cold hardiness; Colorado potato beetles; imidacloprid; next-generation sequencing
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Morin, M.D.; Lyons, P.J.; Crapoulet, N.; Boquel, S.; Morin, P.J. Identification of Differentially Expressed miRNAs in Colorado Potato Beetles (Leptinotarsa decemlineata (Say)) Exposed to Imidacloprid. Int. J. Mol. Sci. 2017, 18, 2728.

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