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Int. J. Mol. Sci. 2018, 19(1), 50; https://doi.org/10.3390/ijms19010050

Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro

1
Division of Applied Life Science (BK21 Plus program), Gyeongsang National University, Jinju 52828, Korea
2
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea
3
Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 10 November 2017 / Revised: 12 December 2017 / Accepted: 22 December 2017 / Published: 24 December 2017
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The present study depicted the role of silicon in limiting the hyperhydricity in shoot cultures of carnation through proteomic analysis. Four-week-old healthy shoot cultures of carnation “Purple Beauty” were sub-cultured on Murashige and Skoog medium followed with four treatments, viz. control (–Si/–Hyperhydricity), hyperhydric with no silicon treatment (–Si/+Hyperhydricity), hyperhydric with silicon treatment (+Si/+Hyperhydricity), and only silicon treated with no hyperhydricity (+Si/–Hyperhydricity). Comparing to control morphological features of hyperhydric carnations showed significantly fragile, bushy and lustrous leaf nature, while Si supply restored these effects. Proteomic investigation revealed that approximately seventy protein spots were differentially expressed under Si and/or hyperhydric treatments and were either up- or downregulated in abundance depending on their functions. Most of the identified protein spots were related to stress responses, photosynthesis, and signal transduction. Proteomic results were further confirmed through immunoblots by selecting specific proteins such as superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), PsaA, and PsbA. Moreover, protein–protein interaction was also performed on differentially expressed protein spots using specific bioinformatic tools. In addition, stress markers were analyzed by histochemical localization of hydrogen peroxide (H2O2) and singlet oxygen (O21–). In addition, the ultrastructure of chloroplasts in hyperhydric leaves significantly resulted in inefficiency of thylakoid lamella with the loss of grana but were recovered in silicon supplemented leaves. The proteomic study together with physiological analysis indicated that Si has a substantial role in upholding the hyperhydricity in in vitro grown carnation shoot cultures. View Full-Text
Keywords: carnation; hyperhydricity; immunoblots; mass spectrometer; proteomics; stress response; silicon carnation; hyperhydricity; immunoblots; mass spectrometer; proteomics; stress response; silicon
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Muneer, S.; Wei, H.; Park, Y.G.; Jeong, H.K.; Jeong, B.R. Proteomic Analysis Reveals the Dynamic Role of Silicon in Alleviation of Hyperhydricity in Carnation Grown In Vitro. Int. J. Mol. Sci. 2018, 19, 50.

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