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Int. J. Mol. Sci. 2018, 19(1), 5; https://doi.org/10.3390/ijms19010005

Pituitary Gonadotropins, Prolactin and Growth Hormone Differentially Regulate AQP1 Expression in the Porcine Ovarian Follicular Cells

1
Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, 10-752 Olsztyn, Poland
2
Department of Human Physiology, University of Warmia and Mazury in Olsztyn, 10-752 Olsztyn, Poland
3
Department of Health Science and Technology, Aalborg University, 9220 Aalborg, Denmark
*
Authors to whom correspondence should be addressed.
Received: 25 October 2017 / Revised: 16 December 2017 / Accepted: 16 December 2017 / Published: 21 December 2017
(This article belongs to the Special Issue Aquaporins: Water Channels Essential for Living Organisms)
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Abstract

The present in vitro study analyzed whether the hormones that affect the ovarian follicular steroidogenesis process also participate in the regulation of AQP1 mRNA and protein expression. Granulosa (Gc) and theca cells (Tc) of medium and large porcine ovarian follicles were exposed to follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL) and growth hormone (GH) for 24 h in separated cells and co-cultures of these cells. Real-time PCR, Western blotting, immunofluorescence and volumetric analysis were then performed. Gonadotropins, PRL and GH had a stimulatory impact on AQP1 mRNA and protein expression in Gc and Tc of medium and large ovarian cells. Moreover, swelling assays, in response to a hypotonic environment, demonstrated the functional presence of AQPs in porcine Gc and Tc. Immunofluorescence analysis showed that AQP1 protein was mainly localized in the perinuclear region of the cytoplasm, endosomes and cell membranes of Gc and Tc from medium and large follicles. It seems possible that AQP1 present in Gc and Tc cells may be implicated not only in the regulation of water homeostasis required for follicle development but also in cell proliferation and migration. View Full-Text
Keywords: pig; ovarian follicles; pituitary hormones; aquaporin 1; in vitro study pig; ovarian follicles; pituitary hormones; aquaporin 1; in vitro study
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Skowronski, M.T.; Mlotkowska, P.; Tanski, D.; Lepiarczyk, E.; Oklinski, M.K.; Nielsen, S.; Skowronska, A. Pituitary Gonadotropins, Prolactin and Growth Hormone Differentially Regulate AQP1 Expression in the Porcine Ovarian Follicular Cells. Int. J. Mol. Sci. 2018, 19, 5.

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