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Int. J. Mol. Sci. 2018, 19(1), 38; https://doi.org/10.3390/ijms19010038

Recombinant Zika NS1 Protein Secreted from Vero Cells Is Efficient for Inducing Production of Immune Serum Directed against NS1 Dimer

1
Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion, INSERM UMR 1187, CNRS 9192, IRD 249, Platform CYROI, 2 rue Maxime Rivière, 97491 Sainte-Clotilde, La Réunion, France
2
Section des Sciences Pharmaceutiques, Université de Genève, rue Michel-Servet 1, 1211 Genève, Switzerland
3
Virologie Moléculaire et Vaccinologie, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris CEDEX 15, France
*
Authors to whom correspondence should be addressed.
Received: 2 November 2017 / Revised: 19 December 2017 / Accepted: 21 December 2017 / Published: 23 December 2017
(This article belongs to the Special Issue Recombinant Proteins)
View Full-Text   |   Download PDF [5263 KB, uploaded 23 December 2017]   |  

Abstract

Zika virus (ZIKV) is a mosquito-borne flavivirus that recently emerged in the South Pacific, Americas, and Caribbean islands, where the larger epidemics were documented. ZIKV infection in humans is responsible for neurological disorders and microcephaly. Flavivirus NS1 is a non-structural glycoprotein that is expressed on the cell surface and secreted as a hexameric lipoprotein particle. Intracellular NS1 exists as a dimer that is required for viral replication, whereas the secreted NS1 hexamer interacts with host factors, leading to pathophysiological conditions. In an effort to dispose of specific anti-ZIKV NS1 immune serum, Vero cells were transduced with a lentiviral vector containing the NS1 gene from an epidemic strain of ZIKV. We showed that stably transduced Vero/ZIKV NS1 cell clone was efficient in the secretion of recombinant NS1 oligomer. Immunization of adult rat with purified extracellular NS1 developed anti-ZIKV antibodies that specifically react with the NS1 dimer produced in human cells infected with African and Asian strains of ZIKV. The rat antibody against ZIKV NS1 dimer is a reliable biological tool that enables the immunological detection of secreted NS1 from host-cells infected with ZIKV. View Full-Text
Keywords: emerging disease; arbovirus; Zika virus; NS1 protein; lentiviral vector; recombinant antigen; humoral immunity; NS1 antiserum emerging disease; arbovirus; Zika virus; NS1 protein; lentiviral vector; recombinant antigen; humoral immunity; NS1 antiserum
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Viranaicken, W.; Ndebo, A.; Bos, S.; Souque, P.; Gadea, G.; El-Kalamouni, C.; Krejbich-Trotot, P.; Charneau, P.; Desprès, P.; Roche, M. Recombinant Zika NS1 Protein Secreted from Vero Cells Is Efficient for Inducing Production of Immune Serum Directed against NS1 Dimer. Int. J. Mol. Sci. 2018, 19, 38.

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