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Int. J. Mol. Sci. 2018, 19(1), 208; https://doi.org/10.3390/ijms19010208

Aeginetia indica Decoction Inhibits Hepatitis C Virus Life Cycle

Institute of Biochemistry and Molecular Biology, National Taiwan University College of Medicine, Taipei 10051, Taiwan
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Received: 13 December 2017 / Revised: 3 January 2018 / Accepted: 5 January 2018 / Published: 9 January 2018
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Abstract

Chronic hepatitis C virus (HCV) infection is still a global epidemic despite the introduction of several highly effective direct-acting antivirals that are tagged with sky-high prices. The present study aimed to identify an herbal decoction that ameliorates HCV infection. Among six herbal decoctions tested, the Aeginetia indica decoction had the most profound effect on the HCV reporter activity in infected Huh7.5.1 liver cells in a dose- and time-dependent manner. The Aeginetia indica decoction exerted multiple inhibitory effects on the HCV life cycle. Pretreatment of the cells with the Aeginetia indica decoction prior to HCV infection reduced the HCV RNA and non-structural protein 3 (NS3) protein levels in the infected cells. The Aeginetia indica decoction reduced HCV internal ribosome entry site-mediated protein translation activity. It also reduced the HCV RNA level in the infected cells in association with reduced NS5A phosphorylation at serine 235, a predominant phosphorylation event indispensable to HCV replication. Thus, the Aeginetia indica decoction inhibits HCV infection, translation, and replication. Mechanistically, the Aeginetia indica decoction probably reduced HCV replication via reducing NS5A phosphorylation at serine 235. View Full-Text
Keywords: herbal decoction; bioactive phytochemical; nutraceutical; alternative medicine; Aeginetia indica; hepatitis C virus (HCV); serine 235; non-structural protein 5A (NS5A); phosphorylation herbal decoction; bioactive phytochemical; nutraceutical; alternative medicine; Aeginetia indica; hepatitis C virus (HCV); serine 235; non-structural protein 5A (NS5A); phosphorylation
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Lin, C.-W.; Lo, C.-W.; Tsai, C.-N.; Pan, T.-C.; Chen, P.-Y.; Yu, M.-J. Aeginetia indica Decoction Inhibits Hepatitis C Virus Life Cycle. Int. J. Mol. Sci. 2018, 19, 208.

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