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Int. J. Mol. Sci. 2018, 19(1), 175; https://doi.org/10.3390/ijms19010175

Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by High Light with Acetate and Fe2+

Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China (OUC), Qingdao 266003, Shandong, China
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Received: 5 December 2017 / Revised: 26 December 2017 / Accepted: 4 January 2018 / Published: 7 January 2018
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Haematococcus pluvialis is a commercial microalga, that produces abundant levels of astaxanthin under stress conditions. Acetate and Fe2+ are reported to be important for astaxanthin accumulation in H. pluvialis. In order to study the synergistic effects of high light stress and these two factors, we obtained transcriptomes for four groups: high light irradiation (HL), addition of 25 mM acetate under high light (HA), addition of 20 μM Fe2+ under high light (HF) and normal green growing cells (HG). Among the total clean reads of the four groups, 156,992 unigenes were found, of which 48.88% were annotated in at least one database (Nr, Nt, Pfam, KOG/COG, SwissProt, KEGG, GO). The statistics for DEGs (differentially expressed genes) showed that there were more than 10 thousand DEGs caused by high light and 1800–1900 DEGs caused by acetate or Fe2+. The results of DEG analysis by GO and KEGG enrichments showed that, under the high light condition, the expression of genes related to many pathways had changed, such as the pathway for carotenoid biosynthesis, fatty acid elongation, photosynthesis-antenna proteins, carbon fixation in photosynthetic organisms and so on. Addition of acetate under high light significantly promoted the expression of key genes related to the pathways for carotenoid biosynthesis and fatty acid elongation. Furthermore, acetate could obviously inhibit the expression of genes related to the pathway for photosynthesis-antenna proteins. For addition of Fe2+, the genes related to photosynthesis-antenna proteins were promoted significantly and there was no obvious change in the gene expressions related to carotenoid and fatty acid synthesis. View Full-Text
Keywords: Haematococcus pluvialis; astaxanthin; high light stress; salt stress; transcriptome Haematococcus pluvialis; astaxanthin; high light stress; salt stress; transcriptome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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He, B.; Hou, L.; Dong, M.; Shi, J.; Huang, X.; Ding, Y.; Cong, X.; Zhang, F.; Zhang, X.; Zang, X. Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by High Light with Acetate and Fe2+. Int. J. Mol. Sci. 2018, 19, 175.

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