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Int. J. Mol. Sci. 2018, 19(1), 140; https://doi.org/10.3390/ijms19010140

Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.)

State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China
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Received: 22 November 2017 / Revised: 21 December 2017 / Accepted: 29 December 2017 / Published: 3 January 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Premature leaf senescence negatively impacts the grain yield in the important monocot rice (Oryza sativa L.); to understand the molecular mechanism we carried out a screen for mutants with premature senescence leaves in a mutant bank generated by ethyl methane sulfonate (EMS) mutagenesis of elite indica rice ZhongJian100. Five premature senescence leaf (psl15, psl50, psl89, psl117 and psl270) mutants were identified with distinct yellowish phenotypes on leaves starting from the tillering stage to final maturation. Moreover, these mutants exhibited significantly increased malonaldehyde content, decreased chlorophyll content, reduced numbers of chloroplast and grana thylakoid, altered photosynthetic ability and expression of photosynthesis-related genes. Furthermore, the expression of senescence-related indicator OsI57 was significantly up-regulated in four mutants. Histochemical analysis indicated that cell death and reactive oxygen species (ROS) accumulation occurred in the mutants with altered activities of ROS scavenging enzymes. Both darkness and abscisic acid (ABA) treatments could induce leaf senescence and resulted in up- or down-regulation of ABA metabolism-related genes in the mutants. Genetic analysis indicated that all the premature senescence leaf mutants were controlled by single non-allelic recessive genes. The data suggested that mechanisms underlying premature leaf senescence are likely different among the mutants. The present study would facilitate us to further fine mapping, cloning and functional characterization of the corresponding genes mediating the premature leaf senescence in rice. View Full-Text
Keywords: premature senescence; chlorophyll; abscisic acid; reactive oxygen species; ultrastructure; rice (Oryza sativa L.) premature senescence; chlorophyll; abscisic acid; reactive oxygen species; ultrastructure; rice (Oryza sativa L.)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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He, Y.; Li, L.; Zhang, Z.; Wu, J.-L. Identification and Comparative Analysis of Premature Senescence Leaf Mutants in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2018, 19, 140.

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