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Int. J. Mol. Sci. 2018, 19(1), 103; https://doi.org/10.3390/ijms19010103

SLC9A3 Protein Is Critical for Acrosomal Formation in Postmeiotic Male Germ Cells

1
Department of Chemistry, Fu Jen Catholic University, New Taipei City 242, Taiwan
2
Graduate Institute of Biomedical and Pharmaceutical Science, Fu Jen Catholic University, New Taipei City 242, Taiwan
3
School of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan
4
Department of Urology, Mackay Memorial Hospital, Taipei 104, Taiwan
5
Department of Pathology, Cardinal Tien Hospital, New Taipei City 242, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 6 October 2017 / Revised: 13 December 2017 / Accepted: 19 December 2017 / Published: 29 December 2017
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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Abstract

Solute carrier family 9 isoform 3 (SLC9A3), a Na+/H+ exchanger, regulates the transepithelial absorption of Na+ and water and is primarily expressed on the apical membranes of the intestinal epithelium, renal proximal tubule, epididymis, and vas deferens. Loss of the Slc9a3 allele in mice enhances intestinal fluid and causes diarrhoea as a consequence of diminished Na+ and HCO3 absorption. Hence, the loss also causes male infertility and reveals the abnormal dilated lumen of the rete testis and calcification in efferent ductules. However, whether loss of Slc9a3 alleles also disrupts mammalian spermatogenesis remains unknown. First, through immunoblotting, we determined that SLC9A3 is highly expressed in the murine testis compared with the small intestine, epididymis, and vas deferens. During murine spermatogenesis, SLC9A3 is specifically expressed in the acrosome region of round, elongating, and elongated spermatids through immunostaining. Furthermore, SLC9A3 signals are enriched in the acrosome of mature sperm isolated from the vas deferens. In Slc9a3 knockout (KO) mice, compared with the same-aged controls, the number of spermatids on the testicular section of the mice progressively worsened in mice aged 20, 35, and 60 days. Sperm isolated from the epididymis of Slc9a3 KO mice revealed severe acrosomal defects. Our data indicated that SLC9A3 has a vital role in acrosomal formation during spermiogenesis. View Full-Text
Keywords: SLC9A3; knockout mice; acrosome SLC9A3; knockout mice; acrosome
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Wang, Y.-Y.; Chiang, H.-S.; Cheng, C.-Y.; Wu, Y.-N.; Lin, Y.-C.; Liu, H.-C.; Tsai, W.-K.; Chen, Y.-L.; Lin, Y.-H. SLC9A3 Protein Is Critical for Acrosomal Formation in Postmeiotic Male Germ Cells. Int. J. Mol. Sci. 2018, 19, 103.

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