Next Article in Journal
The Role of ERK Signaling in Experimental Autoimmune Encephalomyelitis
Previous Article in Journal
Genome Instability and γH2AX
Previous Article in Special Issue
The Actin Cytoskeleton Is Involved in Glial Cell Line-Derived Neurotrophic Factor (GDNF)-Induced Ret Translocation into Lipid Rafts in Dopaminergic Neuronal Cells
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessCommunication
Int. J. Mol. Sci. 2017, 18(9), 1987; doi:10.3390/ijms18091987

Effect of Clotting Duration and Temperature on BDNF Measurement in Human Serum

1
Centro Cardiologico Monzino, IRCCS, 20138 Milan, Italy
2
Department of Pharmacological and Biomolecular Sciences, University of Milan, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
Received: 11 August 2017 / Revised: 29 August 2017 / Accepted: 2 September 2017 / Published: 15 September 2017
(This article belongs to the Special Issue Brain-Derived Neurotrophic Factor)
View Full-Text   |   Download PDF [949 KB, uploaded 15 September 2017]   |  

Abstract

Brain-derived neurothrophic factor (BDNF) is a neurotrophin expressed in different tissues and cells, including neurons, endothelial cells, leukocytes, megakaryocytes and platelets. Modifications of BDNF in plasma and/or in serum are associated with neurodegenerative and psychiatric disorders, cardiovascular diseases, metabolic syndrome and with mortality risk. Indeed, changes in blood levels of BDNF may reflect those of its tissue of origin and/or promote pathological dysfunctions. The measurement of BDNF amount in plasma or in serum has been characterized with particular attention in the impact of different anti-coagulants, clotting duration, temperature (≤21 °C) and delay in blood sample centrifugation as well as in stability of storage. However, the influences of normothermic conditions (37 °C) and of clotting duration on BDNF levels in human serum have not been investigated yet. Here, we showed that time and temperature during serum preparation could be taken into consideration to assess the association and/or impact of BDNF levels in the occurrence of pathological conditions. View Full-Text
Keywords: BDNF; temperature; clotting duration; serum BDNF; temperature; clotting duration; serum
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Amadio, P.; Sandrini, L.; Ieraci, A.; Tremoli, E.; Barbieri, S.S. Effect of Clotting Duration and Temperature on BDNF Measurement in Human Serum. Int. J. Mol. Sci. 2017, 18, 1987.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top