Next Article in Journal
X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature
Next Article in Special Issue
Effect of Clotting Duration and Temperature on BDNF Measurement in Human Serum
Previous Article in Journal
Implications of ABCG2 Expression on Irinotecan Treatment of Colorectal Cancer Patients: A Review
Previous Article in Special Issue
Salivary Gland Derived BDNF Overexpression in Mice Exerts an Anxiolytic Effect
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(9), 1922; doi:10.3390/ijms18091922

The Actin Cytoskeleton Is Involved in Glial Cell Line-Derived Neurotrophic Factor (GDNF)-Induced Ret Translocation into Lipid Rafts in Dopaminergic Neuronal Cells

1
Department of Pathophysiology, School of the Basic Medicine, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China
2
Institute of Emergency Rescue Medicine, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China
3
School of Nursing, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China
4
Department of Histology and Embryology, School of the Basic Medicine, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China
5
Department of Neurobiology and Anatomy, Xuzhou Key Laboratory of Neurobiology, Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China
6
Institute of Medical Technology, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China
*
Author to whom correspondence should be addressed.
Received: 6 July 2017 / Revised: 25 August 2017 / Accepted: 5 September 2017 / Published: 7 September 2017
(This article belongs to the Special Issue Brain-Derived Neurotrophic Factor)
View Full-Text   |   Download PDF [2935 KB, uploaded 7 September 2017]   |  

Abstract

Glial cell line-derived neurotrophic factor (GDNF), a potential therapeutic factor for Parkinson’s disease (PD), exerts its biological effects through the Ret receptor tyrosine kinase. The redistribution of Ret into lipid rafts substantially influences Ret signaling, but the mechanisms underlying Ret translocation remain unclear. The purpose of our study was to further explore the signaling mechanisms of GDNF and to determine whether the actin cytoskeleton is involved in the GDNF-induced Ret translocation into lipid rafts. In MN9D dopaminergic neuronal cells, we used density gradient centrifugation and immunofluorescence confocal microscopy to separate and visualize lipid rafts, co-immunoprecipitation to analyze protein-protein interactions, and latrunculin B (Lat B) and jasplakinolide (Jas) to disrupt and enhance the polymerization of the actin cytoskeleton, respectively. The results showed that Ret translocated into lipid rafts and coimmunoprecipitated with actin in response to GDNF treatment. After Lat B or Jas treatment, the Ret–F-actin association induced by GDNF was impaired or enhanced respectively and then the levels of Ret translocated into lipid rafts were correspondingly inhibited or promoted. These data indicate that actin polymerization and cytoskeletal remodeling are integral to GDNF-induced cell signaling in dopaminergic cells and define a new role of the actin cytoskeleton in promoting Ret redistribution into lipid rafts. View Full-Text
Keywords: GDNF; Ret; dopaminergic cells; lipid rafts; cytoskeleton GDNF; Ret; dopaminergic cells; lipid rafts; cytoskeleton
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Li, L.; Song, H.; Mu, P.; Xu, M.; Liu, C.; Wang, Y.; Qin, Y.; Sun, S.; Gao, J.; Wang, T.; Gao, D. The Actin Cytoskeleton Is Involved in Glial Cell Line-Derived Neurotrophic Factor (GDNF)-Induced Ret Translocation into Lipid Rafts in Dopaminergic Neuronal Cells. Int. J. Mol. Sci. 2017, 18, 1922.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top