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Int. J. Mol. Sci. 2017, 18(9), 1855; doi:10.3390/ijms18091855

Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor

Laboratory of Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University, Sapporo 0010021, Japan
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Received: 16 July 2017 / Revised: 18 August 2017 / Accepted: 22 August 2017 / Published: 25 August 2017
(This article belongs to the Section Molecular Biophysics)
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Abstract

The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively characterize the diffusion properties of EGFP labeled human GR (EGFP-hGR) and its mutants in the cell nucleus. RICS is an image correlation technique that evaluates the spatial distribution of the diffusion coefficient as a diffusion map. Interestingly, we observed that the averaged diffusion coefficient of EGFP-hGR strongly and negatively correlated with its transcriptional activities in comparison to that of EGFP-hGR wild type and mutants with various transcriptional activities. This result suggests that the decreasing of the diffusion coefficient of hGR was reflected in the high-affinity binding to DNA. Moreover, the hyper-phosphorylation of hGR can enhance the transcriptional activity by reduction of the interaction between the hGR and the nuclear corepressors. View Full-Text
Keywords: raster image correlation spectroscopy; green fluorescence protein; nuclear receptor; diffusion analysis; transcription raster image correlation spectroscopy; green fluorescence protein; nuclear receptor; diffusion analysis; transcription
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Mikuni, S.; Yamamoto, J.; Horio, T.; Kinjo, M. Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor. Int. J. Mol. Sci. 2017, 18, 1855.

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