Phellinus linteus Grown on Germinated Brown Rice Increases Cetuximab Sensitivity of KRAS-Mutated Colon Cancer
Abstract
:1. Introduction
2. Results
2.1. Phellinus Linteus on Germinated Brown Rice (PBR) Inhibits Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS)-Mutated Colon Cancer Cell Proliferation
2.1.1. PBR Extract Increases the Sensitivity of a KRAS-Mutated Colon Cancer Cell Line to Cetuximab
2.1.2. Cetuximab and PBR Extract Co-Treatment Alters Cell Morphology and Clonogenic Characteristics
2.1.3. PBR ExtractInduced KRAS-Mutated Colon Cancer Cell Apoptosis
2.2. Effect of the Combination Treatment of PBR Extract and Cetuximab on KRAS and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways
2.3. PBR Extract Inhibits Xenografted Colon Cancer Growth In Vivo
3. Discussion
4. Materials and Methods
4.1. Preparation of PBR Extract
4.2. Cell Viability by MTS Proliferation Assay and DAPI/PI Staining
4.3. Live/Dead Staining
4.4. Clonogenic Assay
4.5. Flow Cytometry
4.6. Western Blotting
4.7. Animal Experiment
4.8. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Park, H.-J.; Park, J.-B.; Lee, S.-J.; Song, M. Phellinus linteus Grown on Germinated Brown Rice Increases Cetuximab Sensitivity of KRAS-Mutated Colon Cancer. Int. J. Mol. Sci. 2017, 18, 1746. https://doi.org/10.3390/ijms18081746
Park H-J, Park J-B, Lee S-J, Song M. Phellinus linteus Grown on Germinated Brown Rice Increases Cetuximab Sensitivity of KRAS-Mutated Colon Cancer. International Journal of Molecular Sciences. 2017; 18(8):1746. https://doi.org/10.3390/ijms18081746
Chicago/Turabian StylePark, Hye-Jin, Jeong-Bin Park, Sang-Jae Lee, and Minjung Song. 2017. "Phellinus linteus Grown on Germinated Brown Rice Increases Cetuximab Sensitivity of KRAS-Mutated Colon Cancer" International Journal of Molecular Sciences 18, no. 8: 1746. https://doi.org/10.3390/ijms18081746