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Int. J. Mol. Sci. 2017, 18(8), 1708; doi:10.3390/ijms18081708

Co-Infection of Mosquitoes with Chikungunya and Dengue Viruses Reveals Modulation of the Replication of Both Viruses in Midguts and Salivary Glands of Aedes aegypti Mosquitoes

1
Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur, 75015 Paris, France
2
Imagopole, Institut Pasteur, 75015 Paris, France
3
Unité de Recherche et d’Expertise Histotechnologie et Pathologie, Institut Pasteur, 75015 Paris, France
4
Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok 73170, Thailand
5
Interspecies transmission of arboviruses and Therapeutics research Unit, Institut Pasteur of Shanghai, Shanghai Chinese Academy of Sciences, Shanghai 200031, China
6
Maladies infectieuses et vecteurs: écologie, génétique, évolution et contrôle (MIVEGEC), IRD, 34394 Montpellier, France
7
Unité Environnement et Risques Infectieux, Institut Pasteur, 75015 Paris, France
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 21 June 2017 / Revised: 19 July 2017 / Accepted: 28 July 2017 / Published: 4 August 2017
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Abstract

Arthropod-borne virus (arbovirus) infections cause several emerging and resurgent infectious diseases in humans and animals. Chikungunya-affected areas often overlap with dengue-endemic areas. Concurrent dengue virus (DENV) and chikungunya virus (CHIKV) infections have been detected in travelers returning from regions of endemicity. CHIKV and DENV co-infected Aedes albopictus have also been collected in the vicinity of co-infected human cases, emphasizing the need to study co-infections in mosquitoes. We thus aimed to study the pathogen-pathogen interaction involved in these co-infections in DENV/CHIKV co-infected Aedes aegypti mosquitoes. In mono-infections, we detected CHIKV antigens as early as 4 days post-virus exposure in both the midgut (MG) and salivary gland (SG), whereas we detected DENV serotype 2 (DENV-2) antigens from day 5 post-virus exposure in MG and day 10 post-virus exposure in SG. Identical infection rates were observed for singly and co-infected mosquitoes, and facilitation of the replication of both viruses at various times post-viral exposure. We observed a higher replication for DENV-2 in SG of co-infected mosquitoes. We showed that mixed CHIKV and DENV infection facilitated viral replication in Ae. aegypti. The outcome of these mixed infections must be further studied to increase our understanding of pathogen-pathogen interactions in host cells. View Full-Text
Keywords: Aedes aegypti; arbovirus; chikungunya; dengue; co-infection Aedes aegypti; arbovirus; chikungunya; dengue; co-infection
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Le Coupanec, A.; Tchankouo-Nguetcheu, S.; Roux, P.; Khun, H.; Huerre, M.; Morales-Vargas, R.; Enguehard, M.; Lavillette, D.; Missé, D.; Choumet, V. Co-Infection of Mosquitoes with Chikungunya and Dengue Viruses Reveals Modulation of the Replication of Both Viruses in Midguts and Salivary Glands of Aedes aegypti Mosquitoes. Int. J. Mol. Sci. 2017, 18, 1708.

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