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Int. J. Mol. Sci. 2017, 18(7), 1499; doi:10.3390/ijms18071499

Entrainment of Breast Cell Lines Results in Rhythmic Fluctuations of MicroRNAs

1
Grupo de Investigación en Terapia Celular y Medicina Regenerativa, Escuela de Medicina, Tecnológico de Monterrey, Monterrey NL 64710, Mexico
2
Cienciamed Company, San Pedro Garza García NL 66278, Mexico
3
Grupo de Investigación en Bioinformática, Escuela de Medicina, Tecnológico de Monterrey, Monterrey NL 64710, Mexico
*
Author to whom correspondence should be addressed.
Received: 17 May 2017 / Revised: 12 June 2017 / Accepted: 5 July 2017 / Published: 12 July 2017
(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Circadian Rhythms)
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Abstract

Circadian rhythms are essential for temporal (~24 h) regulation of molecular processes in diverse species. Dysregulation of circadian gene expression has been implicated in the pathogenesis of various disorders, including hypertension, diabetes, depression, and cancer. Recently, microRNAs (miRNAs) have been identified as critical modulators of gene expression post-transcriptionally, and perhaps involved in circadian clock architecture or their output functions. The aim of the present study is to explore the temporal expression of miRNAs among entrained breast cell lines. For this purpose, we evaluated the temporal (28 h) expression of 2006 miRNAs in MCF-10A, MCF-7, and MDA-MB-231 cells using microarrays after serum shock entrainment. We noted hundreds of miRNAs that exhibit rhythmic fluctuations in each breast cell line, and some of them across two or three cell lines. Afterwards, we validated the rhythmic profiles exhibited by miR-141-5p, miR-1225-5p, miR-17-5p, miR-222-5p, miR-769-3p, and miR-548ay-3p in the above cell lines, as well as in ZR-7530 and HCC-1954 using RT-qPCR. Our results show that serum shock entrainment in breast cells lines induces rhythmic fluctuations of distinct sets of miRNAs, which have the potential to be related to endogenous circadian clock, but extensive investigation is required to elucidate that connection. View Full-Text
Keywords: circadian rhythms; rhythmic fluctuations microRNAs; microarrays; RT-qPCR; breast cancer circadian rhythms; rhythmic fluctuations microRNAs; microarrays; RT-qPCR; breast cancer
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chacolla-Huaringa, R.; Moreno-Cuevas, J.; Trevino, V.; Scott, S.-P. Entrainment of Breast Cell Lines Results in Rhythmic Fluctuations of MicroRNAs. Int. J. Mol. Sci. 2017, 18, 1499.

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