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Int. J. Mol. Sci. 2017, 18(7), 1493; doi:10.3390/ijms18071493

The SBP-Box Gene VpSBP11 from Chinese Wild Vitis Is Involved in Floral Transition and Affects Leaf Development

1,2,3,†
,
1,2,†
,
3
,
1,2
and
1,2,*
1
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China
2
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling 712100, China
3
College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 6 June 2017 / Revised: 3 July 2017 / Accepted: 6 July 2017 / Published: 13 July 2017
(This article belongs to the Section Molecular Botany)
View Full-Text   |   Download PDF [5547 KB, uploaded 13 July 2017]   |  

Abstract

Flowering occurs in angiosperms during a major developmental transition from vegetative growth to the reproductive phase. Squamosa promoter binding protein (SBP)-box genes have been found to play critical roles in regulating flower and fruit development, but their roles in grapevine have remained unclear. To better understand the functions of the grape SBP-box genes in both vegetative and reproductive growth phases, a full-length complementary DNA (cDNA) sequence of the putative SBP-box transcription factor gene, VpSBP11, was obtained from Chinese wild grapevine Vitis pseudoreticulata Wen Tsai Wang (W. T. Wang) clone ‘Baihe-35-1’. VpSBP11 encoded a putative polypeptide of 170 amino acids with a highly conserved SBP-domain with two zinc-binding sites of the Cx2C-x3-H-x11-C-x6-H (C2HCH) type and a nuclear localization signal. We confirmed that the VpSBP11 protein was targeted to the nucleus and possessed transcriptional activation activity by subcellular localization and trans-activation assay. Over-expression of VpSBP11 in Arabidopsis thaliana was shown to activate the FUL gene, and subsequently the AP1 and LFY genes, all of which were floral meristem identity genes, and to cause earlier flowering than in wild type (WT) plants. The pattern of vegetative growth was also different between the transgenic and WT plants. For example, in the VpSBP11 over-expressing transgenic plants, the number of rosette leaves was less than that of WT; the petiole was significantly elongated; and the rosette and cauline leaves curled upwards or downwards. These results were consistent with VpSBP11 acting as a transcription factor during the transition from the vegetative stage to the reproductive stage. View Full-Text
Keywords: Vitis pseudoreticulata; VpSBP11; floral transition Vitis pseudoreticulata; VpSBP11; floral transition
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Hou, H.; Yan, X.; Sha, T.; Yan, Q.; Wang, X. The SBP-Box Gene VpSBP11 from Chinese Wild Vitis Is Involved in Floral Transition and Affects Leaf Development. Int. J. Mol. Sci. 2017, 18, 1493.

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